Your browser doesn't support javascript.
loading
Development of an efficient, effective, and economical technology for proteome analysis.
Martin, Katherine R; Le, Ha T; Abdelgawad, Ahmed; Yang, Canyuan; Lu, Guotao; Keffer, Jessica L; Zhang, Xiaohui; Zhuang, Zhihao; Asare-Okai, Papa Nii; Chan, Clara S; Batish, Mona; Yu, Yanbao.
Afiliación
  • Martin KR; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
  • Le HT; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
  • Abdelgawad A; Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA; Department of Medical and Molecular Sciences, University of Delaware, Newark, DE 19716, USA.
  • Yang C; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
  • Lu G; CDS Analytical, LLC, Oxford, PA 19363, USA.
  • Keffer JL; Department of Earth Sciences, University of Delaware, Newark, DE 19716, USA.
  • Zhang X; CDS Analytical, LLC, Oxford, PA 19363, USA.
  • Zhuang Z; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
  • Asare-Okai PN; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
  • Chan CS; Department of Earth Sciences, University of Delaware, Newark, DE 19716, USA; School of Marine Science and Policy, University of Delaware, Newark, DE 19716, USA.
  • Batish M; Department of Biological Sciences, University of Delaware, Newark, DE 19716, USA; Department of Medical and Molecular Sciences, University of Delaware, Newark, DE 19716, USA. Electronic address: batish@udel.edu.
  • Yu Y; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA. Electronic address: yybyu@udel.edu.
Cell Rep Methods ; 4(6): 100796, 2024 Jun 17.
Article en En | MEDLINE | ID: mdl-38866007
ABSTRACT
We present an efficient, effective, and economical approach, named E3technology, for proteomics sample preparation. By immobilizing silica microparticles into the polytetrafluoroethylene matrix, we develop a robust membrane medium, which could serve as a reliable platform to generate proteomics-friendly samples in a rapid and low-cost fashion. We benchmark its performance using different formats and demonstrate them with a variety of sample types of varied complexity, quantity, and volume. Our data suggest that E3technology provides proteome-wide identification and quantitation performance equivalent or superior to many existing methods. We further propose an enhanced single-vessel approach, named E4technology, which performs on-filter in-cell digestion with minimal sample loss and high sensitivity, enabling low-input and low-cell proteomics. Lastly, we utilized the above technologies to investigate RNA-binding proteins and profile the intact bacterial cell proteome.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteoma / Proteómica Idioma: En Revista: Cell Rep Methods Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteoma / Proteómica Idioma: En Revista: Cell Rep Methods Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos