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Automated online solid-phase extraction-tandem mass spectrometry detection for simultaneous analysis of acidic and alkaline catecholamines and their metabolites in human urine.
Deng, Shunyan; Wang, Yu; Huang, Xia; Zhou, Yan; Wang, Tianxun; Chen, Xiquan; Xiong, Lan; Wu, Wenlin; Xia, Bing.
Afiliación
  • Deng S; Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 101408, China.
  • Wang Y; Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 101408, China.
  • Huang X; Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 101408, China.
  • Zhou Y; Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
  • Wang T; The First People's Hospital of Guangyuan, Guangyuan 628000, China.
  • Chen X; The First People's Hospital of Guangyuan, Guangyuan 628000, China.
  • Xiong L; The First People's Hospital of Guangyuan, Guangyuan 628000, China.
  • Wu W; Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 101408, China; Chengdu Institute of Food Inspection, Chengdu 611130, China; Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Mar
  • Xia B; Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. Electronic address: xiabing@cib.ac.cn.
J Pharm Biomed Anal ; 248: 116292, 2024 Sep 15.
Article en En | MEDLINE | ID: mdl-38865926
ABSTRACT
Metabolic dysregulation of catecholamines (CAs) is implicated in various human diseases. Simultaneously analyzing these acidic and alkaline CAs and their metabolites poses a significant challenge for clinical detection. This study introduces an efficient method employing automated online solid-phase extraction coupled with tandem mass spectrometry (aoSPE-MS/MS). The method employs weak cation exchange (WCX) and mixed-mode anion exchange (MAX) adsorbents to fabricate an on-line solid-phase extraction (SPE) column, along with an automated injection and multi-valve switching capabilities. The setup allows for automated extraction and analysis of urine samples in 15 minutes while retaining a wide range of acidic and basic CAs and their metabolites. The applicability of this method was demonstrated by optimising the adsorbent dosage volume, extraction solvent, and extraction rate. The limits of detection (LODs) and limits of quantitation (LOQs) for the 8 CAs and their metabolites were determined using the aoSPE-MS/MS approach, with ranges of 0.0625 ∼ 62.5 ng/mL and 0.125 ∼ 125 ng/mL, respectively. Additionally, assessments were made on the linearity, accuracy, and precision within and between batches, as well as matrix and ionic effects, and spiked recoveries. The study discovered that the aoSPE-MS/MS technique simplifies operation, increases efficiency, saves time, and has low detection and quantification limits when detecting a wide range of acid and alkaline CAs and their metabolites in urine. The study successfully demonstrated the high-throughput and automated detection of the 8 CAs and their metabolites with varying acidity and alkalinity in human urine samples. This method is expected to be a potential powerful tool for clinical detection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Catecolaminas / Espectrometría de Masas en Tándem / Extracción en Fase Sólida / Límite de Detección Límite: Humans Idioma: En Revista: J Pharm Biomed Anal Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Catecolaminas / Espectrometría de Masas en Tándem / Extracción en Fase Sólida / Límite de Detección Límite: Humans Idioma: En Revista: J Pharm Biomed Anal Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido