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Impact of papain on the treatment of raw diluted dromedary semen.
Abdel-Ghani, Mohammed A; Ghoneim, Ibrahim M; Nagano, Masashi; AlMomen, Hussain Qasem M.
Afiliación
  • Abdel-Ghani MA; Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, AL-Ahsa, Saudi Arabia.
  • Ghoneim IM; Department of Theriogenology, Faculty of Veterinary Medicine, Assuit University, Assuit, Egypt.
  • Nagano M; Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, AL-Ahsa, Saudi Arabia.
  • AlMomen HQM; Laboratory of Animal Reproduction, Department of Animal Science, School of Veterinary Medicine, Kitasato University, Towada, Japan.
Reprod Domest Anim ; 59(6): e14637, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38864674
ABSTRACT
A variety of parameters, including liquefaction and semen viscosity, affect the sperm's ability to travel and reach the egg for fertilization and conception. Given that the details behind the viscosity of the semen in male camels have not yet been fully clarified, the purpose of this study was to ascertain how the addition of papain affected the viscosity of fresh diluted camel semen. The study examined semen samples derived from camels that had distinct viscosities. Sperm motility, viability, abnormal sperm percentage, concentration, viscosity, morphometry, acrosome integrity and liquefaction were among the evaluations following 0, 5, 10, 20 or 30 min of incubation at 37°C with papain (0.004 mg/mL, 0.04 mg/mL or 0.4 mg/mL; a semen sample without papain was used as a control). A statistically significant interaction between the effects of papain concentrations and incubation time was found (F = 41.68, p = .0001). Papain concentrations (p = .0001) and incubation times (p = .0001) both had a statistically significant impact on viscosity, according to a simple main effects analysis. A lower viscosity was found (p < .05) at 0.04 mg/mL (0.1 ± 0.0) after 10 min of incubation. A simple main effects analysis showed that papain concentrations and incubation time have a statistically significant effect on sperm motility (p = .0001). At 0.04 mg/mL papain, the sperm motility % was higher (p < .05) after 10 min (64.4 ± 4.8), 20 min (68.4 ± 6.2), and 30 min incubation (72.2 ± 6.6) compared to 0, 5 min (38.3 ± 4.1 and 51.6 ± 5.0, respectively). In conclusion, the fresh diluted camel semen had the lowest viscosity properties after 10 min of incubation with 0.04 mg/mL papain, without compromising sperm motility, viability, acrosome integrity and sperm morphology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Preservación de Semen / Motilidad Espermática / Camelus / Papaína Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Preservación de Semen / Motilidad Espermática / Camelus / Papaína Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Alemania