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Uricase biofunctionalized plasmonic sensor for uric acid detection with APTES-modified gold nanotopping.
Onifade, Olabisi Abdullahi; Yusairie, Fatin Adriena; Abu Bakar, Muhammad Hafiz; Alresheedi, Mohammed Thamer; Khoon Ng, Eng; Mahdi, Mohd Adzir; Muhammad Noor, Ahmad Shukri.
Afiliación
  • Onifade OA; Department of Computer and Communication Systems Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia; Wireless and Photonics Research Centre of Excellence, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang Selangor, Malaysia. Electronic
  • Yusairie FA; Department of Computer and Communication Systems Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. Electronic address: fatinadriena@gmail.com.
  • Abu Bakar MH; Department of Computer and Communication Systems Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia; Wireless and Photonics Research Centre of Excellence, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang Selangor, Malaysia. Electronic
  • Alresheedi MT; Department of Electrical Engineering, College of Engineering, P.O. Box 800, King Saud University, Riyadh 11421, Kingdom of Saudi Arabia. Electronic address: malresheedi@ksu.edu.sa.
  • Khoon Ng E; Department of Engineering, University of Cambridge, Cambridge CB3 0FA, United Kingdom. Electronic address: ekn27@cam.ac.uk.
  • Mahdi MA; Wireless and Photonics Research Centre of Excellence, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang Selangor, Malaysia; Institute of Nanoscience and Nanotechnology (ION2), Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: mam@upm.edu.my.
  • Muhammad Noor AS; Department of Computer and Communication Systems Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia; Wireless and Photonics Research Centre of Excellence, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang Selangor, Malaysia. Electronic
Biosens Bioelectron ; 261: 116486, 2024 Oct 01.
Article en En | MEDLINE | ID: mdl-38861811
ABSTRACT
Current uric acid detection methodologies lack the requisite sensitivity and selectivity for point-of-care applications. Plasmonic sensors, while promising, demand refinement for improved performance. This work introduces a biofunctionalized sensor predicated on surface plasmon resonance to quantify uric acid within physiologically relevant concentration ranges. The sensor employs the covalent immobilization of uricase enzyme using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) crosslinking agents, ensuring the durable adherence of the enzyme onto the sensor probe. Characterization through atomic force microscopy and Fourier transform infrared spectroscopy validate surface alterations. The Langmuir adsorption isotherm model elucidates binding kinetics, revealing a sensor binding affinity of 298.83 (mg/dL)-1, and a maximum adsorption capacity of approximately 1.0751°. The biofunctionalized sensor exhibits a sensitivity of 0.0755°/(mg/dL), a linear correlation coefficient of 0.8313, and a limit of detection of 0.095 mg/dL. Selectivity tests against potentially competing interferents like glucose, ascorbic acid, urea, D-cystine, and creatinine showcase a significant resonance angle shift of 1.1135° for uric acid compared to 0.1853° for interferents at the same concentration. Significantly, at a low uric acid concentration of 0.5 mg/dL, a distinct shift of 0.3706° was observed, setting it apart from the lower values noticed at higher concentrations for all typical interferent samples. The uricase enzyme significantly enhances plasmonic sensors for uric acid detection, showcasing a seamless integration of optical principles and biological recognition elements. These sensors hold promise as vital tools in clinical and point-of-care settings, offering transformative potential in biosensing technologies and the potential to revolutionize healthcare outcomes in biomedicine.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Urato Oxidasa / Ácido Úrico / Técnicas Biosensibles / Resonancia por Plasmón de Superficie / Enzimas Inmovilizadas / Oro Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Urato Oxidasa / Ácido Úrico / Técnicas Biosensibles / Resonancia por Plasmón de Superficie / Enzimas Inmovilizadas / Oro Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido