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Expression of a single-chain monellin (MNEI) mutant with enhanced stability in transgenic mice milk.
Lu, Rui; Li, Xiaoming; Hu, Jian; Wang, Yancui; Jin, Le.
Afiliación
  • Lu R; Jiangsu Food and Pharmaceutical Science College, Huaian, Jiangsu, China.
  • Li X; Jiangsu Agri-Animal Husbandry Vocational College, Taizhou, Jiangsu, China.
  • Hu J; Jiangsu Food and Pharmaceutical Science College, Huaian, Jiangsu, China.
  • Wang Y; Jiangsu Food and Pharmaceutical Science College, Huaian, Jiangsu, China. 20191002@jsfpc.edu.cn.
  • Jin L; Jiangsu Food and Pharmaceutical Science College, Huaian, Jiangsu, China. 20191014@jsfpc.edu.cn.
Transgenic Res ; 33(4): 211-218, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38858256
ABSTRACT
Monellin is a sweet protein that may be used as a safe and healthy sweetener. However, due to its low stability, the application of monellin is currently very limited. Here, we describe a wild-type, a double-sites mutant (E2N/E23A) and a triple-sites mutant (N14A/E23Q/S76Y) of single-chain monellin (MNEI) expressed in transgenic mice milk. Based on enzyme-linked immunoassay (ELISA), Western blot, and sweetness intensity testing, their sweetness and stability were compared. After boiling for 2 min at different pH conditions (2.5, 5.1, 6.8, and 8.2), N14A/E23Q/S76Y-MNEI showed significantly higher sweetness and stability than the wild-type and E2N/E23A-MNEI. These results suggest that N14A/E23Q/S76Y-MNEI shows remarkable potential as a sweetener in the future.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Edulcorantes / Ratones Transgénicos / Leche Límite: Animals Idioma: En Revista: Transgenic Res Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Edulcorantes / Ratones Transgénicos / Leche Límite: Animals Idioma: En Revista: Transgenic Res Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos