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Next generation sequencing-aided screening, isolation, molecular identification, and antimicrobial potential for bacterial endophytes from the medicinal plant, Elephantorrhiza elephantina.
Tlou, Matsobane; Ndou, Benedict; Mabona, Nokufa; Khwathisi, Adivhaho; Ateba, Collins; Madala, Ntakadzeni; Serepa-Dlamini, Mahloro Hope.
Afiliación
  • Tlou M; Department of Biochemistry, School of Physical and Chemical Sciences, North-West University, Mmabatho, South Africa.
  • Ndou B; Department of Biochemistry, School of Physical and Chemical Sciences, North-West University, Mmabatho, South Africa.
  • Mabona N; Department of Biochemistry, School of Physical and Chemical Sciences, North-West University, Mmabatho, South Africa.
  • Khwathisi A; Department of Biochemistry and Microbiology, University of Venda, Thohoyandou, South Africa.
  • Ateba C; Department of Microbiology, Faculty of Natural and Agricultural Sciences, School of Biological Sciences, North-West University, Mmabatho, South Africa.
  • Madala N; Department of Biochemistry and Microbiology, University of Venda, Thohoyandou, South Africa.
  • Serepa-Dlamini MH; Department of Biotechnology and Food Technology, University of Johannesburg, Doornfontein Campus, Johannesburg, South Africa.
Front Microbiol ; 15: 1383854, 2024.
Article en En | MEDLINE | ID: mdl-38855763
ABSTRACT
Elephantorrhiza elephantina, a wild plant in southern Africa, is utilized in traditional medicine for various ailments, leading to its endangerment and listing on the Red List of South African Plants. To date, there have been no reports on bacterial endophytes from this plant, their classes of secondary metabolites, and potential medicinal properties. This study presents (i) taxonomic characterization of bacterial endophytes in leaf and root tissues using 16S rRNA, (ii) bacterial isolation, morphological, and phylogenetic characterization, (iii) bacterial growth, metabolite extraction, and LC-MS-based metabolite fingerprinting, and (iv) antimicrobial testing of bacterial crude extracts. Next-generation sequencing yielded 693 and 2,459 DNA read counts for the rhizomes and leaves, respectively, detecting phyla including Proteobacteria, Bacteroidota, Gemmatimonadota, Actinobacteriota, Verrucomicrobiota, Dependentiae, Firmicutes, and Armatimonodata. At the genus level, Novosphingobium, Mesorhizobium, Methylobacterium, and Ralstonia were the most dominant in both leaves and rhizomes. From root tissues, four bacterial isolates were selected, and 16S rRNA-based phylogenetic characterization identified two closely related Pseudomonas sp. (strain BNWU4 and 5), Microbacterium oxydans BNWU2, and Stenotrophomonas maltophilia BNWU1. The ethyl acetatechloroform (11 v/v) organic extract from each isolate exhibited antimicrobial activity against all selected bacterial pathogens. Strain BNWU5 displayed the highest activity, with minimum inhibitory concentrations ranging from 62.5 µg/mL to 250 µg/mL against diarrhoeagenic Escherichia coli, Escherichia coli O157H7, Salmonella enterica, antibiotic-resistant Vibrio cholerae, Staphylococcus aureus, Bacillus cereus, and Enterococcus durans. LC-MS analysis of the crude extract revealed common antimicrobial metabolites produced by all isolates, including Phenoxomethylpenicilloyl (penicilloyl V), cis-11-Eicosenamide, 3-Hydroxy-3-phenacyloxindole, and 9-Octadecenamide.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Sudáfrica Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Sudáfrica Pais de publicación: Suiza