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What's the situation with ocular inflammation? A cross-seasonal investigation of proteomic changes in ocular allergy sufferers' tears in Victoria, Australia.
Aydin, Esrin; Nie, Shuai; Azizoglu, Serap; Chong, Luke; Gokhale, Moneisha; Suphioglu, Cenk.
Afiliación
  • Aydin E; NeuroAllergy Research Laboratory (NARL), School of Life and Environmental Sciences, Deakin University, Waurn Ponds, VIC, Australia.
  • Nie S; School of Medicine (Optometry), Deakin University, Waurn Ponds, VIC, Australia.
  • Azizoglu S; Bio21 Mass Spectrometry and Proteomics Facility, Bio21 Molecular Science & Biotechnology Institute, The University of Melbourne, Melbourne, VIC, Australia.
  • Chong L; School of Medicine (Optometry), Deakin University, Waurn Ponds, VIC, Australia.
  • Gokhale M; School of Medicine (Optometry), Deakin University, Waurn Ponds, VIC, Australia.
  • Suphioglu C; School of Medicine (Optometry), Deakin University, Waurn Ponds, VIC, Australia.
Front Immunol ; 15: 1386344, 2024.
Article en En | MEDLINE | ID: mdl-38855108
ABSTRACT

Background:

Ocular allergy (OA) is a localized subset of allergy characterized by ocular surface itchiness, redness and inflammation. Inflammation and eye-rubbing, due to allergy-associated itch, are common in OA sufferers and may trigger changes to the ocular surface biochemistry. The primary aim of this study is to assess the differences in the human tear proteome between OA sufferers and Healthy Controls (HCs) across peak allergy season and off-peak season in Victoria, Australia.

Methods:

19 participants (14 OA sufferers, 5 HCs) aged 18-45 were recruited for this study. Participants were grouped based on allergy symptom assessment questionnaire scoring. Proteins were extracted from human tear samples and were run on an Orbitrap Mass Spectrometer. Peaks were matched to a DIA library. Data was analyzed using the software MaxQuant, Perseus and IBM SPSS.

Results:

1267 proteins were identified in tear samples of OA sufferers and HCs. 23 proteins were differentially expressed between peak allergy season OA suffers vs HCs, and 21 were differentially expressed in off-peak season. Decreased proteins in OA sufferers related to cell structure regulation, inflammatory regulation and antimicrobial regulation. In both seasons, OA sufferers were shown to have increased expression of proteins relating to inflammation, immune responses and cellular development.

Conclusion:

Tear protein identification showed dysregulation of proteins involved in inflammation, immunity and cellular structures. Proteins relating to cellular structure may suggest a possible link between OA-associated itch and the subsequent ocular surface damage via eye-rubbing, while inflammatory and immune protein changes highlight potential diagnostic and therapeutic biomarkers of OA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estaciones del Año / Lágrimas / Proteoma / Proteómica Límite: Adolescent / Adult / Female / Humans / Male / Middle aged País/Región como asunto: Oceania Idioma: En Revista: Front Immunol Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estaciones del Año / Lágrimas / Proteoma / Proteómica Límite: Adolescent / Adult / Female / Humans / Male / Middle aged País/Región como asunto: Oceania Idioma: En Revista: Front Immunol Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Suiza