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Examining DNA Structures with In-droplet Hydrogen/Deuterium Exchange Mass Spectrometry.
Attanayake, Kushani; Mahmud, Sultan; Banerjee, Chandrima; Sharif, Daud; Rahman, Mohammad; Majuta, Sandra; DeBastiani, Anthony; Sultana, Mst Nigar; Foroushani, Samira Hajian; Li, Chong; Li, Peng; Valentine, Stephen J.
Afiliación
  • Attanayake K; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
  • Mahmud S; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
  • Banerjee C; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
  • Sharif D; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
  • Rahman M; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
  • Majuta S; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
  • DeBastiani A; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
  • Sultana MN; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
  • Foroushani SH; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
  • Li C; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
  • Li P; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
  • Valentine SJ; C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
Int J Mass Spectrom ; 4992024 May.
Article en En | MEDLINE | ID: mdl-38854816
ABSTRACT
Capillary vibrating sharp-edge spray ionization (cVSSI) combined with hydrogen/deuterium exchange-mass spectrometry (HDX-MS) has been utilized to characterize different solution-phase DNA conformers including DNA G-quadruplex topologies as well as triplex DNA and duplex DNA. In general, G-quadruplex DNA shows a wide range of protection of hydrogens extending from ~12% to ~21% deuterium incorporation. Additionally, the DNA sequences selected to represent parallel, antiparallel, and hybrid G-quadruplex topologies exhibit slight differences in deuterium uptake levels which appear to loosely relate to overall conformer stability. Notably, the exchange level for one of the hybrid sequence sub topologies of G-quadruplex DNA (24 TTG) is significantly different (compared with the others studied here) despite the DNA sequences being highly comparable. For the quadruplex-forming sequences, correlation analysis suggests protection of base hydrogens involved in tetrad hydrogen bonding. For duplex DNA ~19% deuterium incorporation is observed while only ~16% is observed for triplex DNA. This increased protection of hydrogens may be due to the added backbone scaffolding and Hoogsteen base pairing of the latter species. These experiments lay the groundwork for future studies aimed at determining the structural source of this protection as well as the applicability of the approach for ascertaining different oligonucleotide folds, co-existing conformations, and/or overall conformer flexibility.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int J Mass Spectrom Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int J Mass Spectrom Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos