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Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease.
Yang, Rong; Zhang, Heng; Chen, Si; Lou, Kaibin; Zhou, Meng; Zhang, Mingchao; Lu, Rui; Zheng, Chunxia; Li, Limin; Chen, Qihan; Liu, Zhihong; Zen, Ke; Yuan, Yanggang; Liang, Hongwei.
Afiliación
  • Yang R; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
  • Zhang H; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, China.
  • Chen S; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
  • Lou K; Department of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
  • Zhou M; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
  • Zhang M; Department of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
  • Lu R; National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
  • Zheng C; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
  • Li L; National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
  • Chen Q; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
  • Liu Z; Cancer Center, Faculty of Health Sciences, University of Macau, Macau, SAR, China.
  • Zen K; National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
  • Yuan Y; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
  • Liang H; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, China.
J Extracell Vesicles ; 13(6): e12460, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38853287
ABSTRACT
Migrasomes represent a recently uncovered category of extracellular microvesicles, spanning a diameter range of 500 to 3000 nm. They are emitted by migrating cells and harbour a diverse array of RNAs and proteins. Migrasomes can be readily identified in bodily fluids like serum and urine, rendering them a valuable non-invasive source for disease diagnosis through liquid biopsy. In this investigation, we introduce a streamlined and effective approach for the capture and quantitative assessment of migrasomes, employing wheat germ agglutinin (WGA)-coated magnetic beads and flow cytometry (referred to as WBFC). Subsequently, we examined the levels of migrasomes in the urine of kidney disease (KD) patients with podocyte injury and healthy volunteers using WBFC. The outcomes unveiled a substantial increase in urinary podocyte-derived migrasome concentrations among individuals with KD with podocyte injury compared to the healthy counterparts. Notably, the urinary podocyte-derived migrasomes were found to express an abundant quantity of phospholipase A2 receptor (PLA2R) proteins. The presence of PLA2R proteins in these migrasomes holds promise for serving as a natural antigen for the quantification of autoantibodies against PLA2R in the serum of patients afflicted by membranous nephropathy. Consequently, our study not only pioneers a novel technique for the isolation and quantification of migrasomes but also underscores the potential of urinary migrasomes as a promising biomarker for the early diagnosis of KD with podocyte injury.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Podocitos Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Extracell Vesicles Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Podocitos Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Extracell Vesicles Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos