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Application of a new designed high resolution melting analysis for mycobacterial species identification.
Khosravi, Azar Dokht; Meghdadi, Hossein; Hashemzadeh, Mohammad; Alami, Ameneh; Tabandeh, Mohammad Reza.
Afiliación
  • Khosravi AD; Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
  • Meghdadi H; Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
  • Hashemzadeh M; Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. h.meghdadi20@gmail.com.
  • Alami A; Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. h.meghdadi20@gmail.com.
  • Tabandeh MR; Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
BMC Microbiol ; 24(1): 205, 2024 Jun 08.
Article en En | MEDLINE | ID: mdl-38851713
ABSTRACT
The Non-tuberculous mycobacterial (NTM) isolates should be distinguished from tuberculosis and identified at the species level for choosing an appropriate treatment plan. In this study, two molecular methods were used to differentiate NTM species, including a new designed High Resolution Melting (HRM) and Multilocus Sequence Analysis (MLSA). Seventy-five mycobacterial isolates were evaluated by sequencing four genes ( MLSA) and a HRM assay specifically targeting atpE was designed to rapidly and accurately identify and differentiate mycobacterium species. Out of 70 NTM isolates, 66 (94.3%), 65 (92.9%), 65 (92.9%) and 64 (91.4%) isolates were identified to the species level by PCR of atpE, tuf, rpoB and dnaK genes. We could identify 100% of the isolates to the species level (14 different species) by MLSA. By using HRM assay, all NTM isolates were identified and classified into eight groups, in addition, Mycobacterium tuberculosis and Nocardia were also detected simultaneously. The MLSA technique was able to differentiate all 14 species of NTM isolates. According to the results, the HRM assay is a rapid and beneficial method for identifying NTM, M. tuberculosis (MTB), and Nocardia isolates without sequencing.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tipificación de Secuencias Multilocus Límite: Humans Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tipificación de Secuencias Multilocus Límite: Humans Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido