Pseudomonas putida Dynamics of Adaptation under Prolonged Resource Exhaustion.

Gross, Jonathan; Katz, Sophia; Hershberg, Ruth

Gross, Jonathan; Katz, Sophia; Hershberg, Ruth.

Afiliación

Gross J; Rachel & Menachem Mendelovitch Evolutionary Processes of Mutation & Natural Selection Research Laboratory, Department of Genetics and Developmental Biology, the Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel.
Katz S; Rachel & Menachem Mendelovitch Evolutionary Processes of Mutation & Natural Selection Research Laboratory, Department of Genetics and Developmental Biology, the Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel.
Hershberg R; Rachel & Menachem Mendelovitch Evolutionary Processes of Mutation & Natural Selection Research Laboratory, Department of Genetics and Developmental Biology, the Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel.

Genome Biol Evol ; 16(6)2024 06 04.

Article en En | MEDLINE | ID: mdl-38849986

ABSTRACT

ABSTRACT

Many nonsporulating bacterial species survive prolonged resource exhaustion, by entering a state termed long-term stationary phase. Here, we performed long-term stationary phase evolutionary experiments on the bacterium Pseudomonas putida, followed by whole-genome sequencing of evolved clones. We show that P. putida is able to persist and adapt genetically under long-term stationary phase. We observed an accumulation of mutations within the evolving P. putida populations. Within each population, independently evolving lineages are established early on and persist throughout the 4-month-long experiment. Mutations accumulate in a highly convergent manner, with similar loci being mutated across independently evolving populations. Across populations, mutators emerge, that due to mutations within mismatch repair genes developed a much higher rate of mutation than other clones with which they coexisted within their respective populations. While these general dynamics of the adaptive process are quite similar to those we previously observed in the model bacterium Escherichia coli, the specific loci that are involved in adaptation only partially overlap between P. putida and E. coli.

Asunto(s)

Adaptación Fisiológica; Pseudomonas putida; Pseudomonas putida/genética; Pseudomonas putida/metabolismo; Adaptación Fisiológica/genética; Mutación; Genoma Bacteriano; Evolución Molecular

Palabras clave

adaptation dynamics; bacterial evolution; long-term stationary phase; prolonged resource exhaustion

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adaptación Fisiológica / Pseudomonas putida Idioma: En Revista: Genome Biol Evol Asunto de la revista: BIOLOGIA / BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Reino Unido

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