Your browser doesn't support javascript.
loading
CircPAPPA2 plays a role in preeclampsia pathogenesis via regulation of the miR-942/miR-5006-3p.
Liao, Wenyan; Zeng, Huan; Jiang, Xinmiao; Deng, Xin; Tu, Shun; Lan, Hui; Tang, Lingling; Dong, Weilei; Ding, Chengming.
Afiliación
  • Liao W; Department of Gynaecology and Obstetrics, Hengyang Medical School, The First Affiliated Hospital, University of South China. NO.69, Chuanshan Road, Hengyang, 421001, Hunan, China.
  • Zeng H; Department of Gynaecology and Obstetrics, Hengyang Medical School, The First Affiliated Hospital, University of South China. NO.69, Chuanshan Road, Hengyang, 421001, Hunan, China.
  • Jiang X; Department of Hepatopancreatobiliary Surgery, Hengyang Medical School, The First Affiliated Hospital, University of South China. NO.69, Chuanshan Road, Hengyang, 421001, Hunan, China.
  • Deng X; Department of Hepatopancreatobiliary Surgery, Hengyang Medical School, The First Affiliated Hospital, University of South China. NO.69, Chuanshan Road, Hengyang, 421001, Hunan, China.
  • Tu S; Department of Hepatopancreatobiliary Surgery, Hengyang Medical School, The First Affiliated Hospital, University of South China. NO.69, Chuanshan Road, Hengyang, 421001, Hunan, China.
  • Lan H; Department of Gynaecology and Obstetrics, Hengyang Medical School, The First Affiliated Hospital, University of South China. NO.69, Chuanshan Road, Hengyang, 421001, Hunan, China.
  • Tang L; Department of Gynaecology and Obstetrics, Hengyang Medical School, The First Affiliated Hospital, University of South China. NO.69, Chuanshan Road, Hengyang, 421001, Hunan, China.
  • Dong W; Department of Gynaecology and Obstetrics, Hengyang Medical School, The First Affiliated Hospital, University of South China. NO.69, Chuanshan Road, Hengyang, 421001, Hunan, China. 540325366@qq.com.
  • Ding C; Department of Hepatopancreatobiliary Surgery, Hengyang Medical School, The First Affiliated Hospital, University of South China. NO.69, Chuanshan Road, Hengyang, 421001, Hunan, China. dingchengming83@163.com.
BMC Pregnancy Childbirth ; 24(1): 414, 2024 Jun 07.
Article en En | MEDLINE | ID: mdl-38849756
ABSTRACT
CircRNAs are a class of endogenous non-coding RNAs implicated in the pathogenesis of many pregnancy related diseases, one of which is pre-eclampsia (PE). This study aims to investigate the role of CircPAPPA2 (circbase ID hsa_circ_0015382) in regulating the migration and invasion of trophoblast cells. RNA sequencing was used to identify the differentially expressed circRNAs in placenta of PE and normal pregnant women. Quantitative polymerase chain reaction (qRT-PCR) was used to verify the expression of circPAPPA2 and two miRNAs (miR-942-5p, 5006-3p) in placenta of PE and normal pregnant women. CCK8 and transwell experiments were performed to assess the function of circPAPPA2 in PE development.The interaction between circPAPPA2 and miR-942-5p/miR-5006-3p was verified by dual-luciferase reporter assay. Finally, bioinformatics analyzed with gene ontology, Kyoto Encyclopedia of the target genes. The results showed that the expression of circPAPPA2 was increased in placenta of PE pregnant women. Also, circPAPPA2 impedes trophoblasts cell proliferation and invasion. Moreover, the expression of circPAPPA2 was positively correlated with systolic blood pressure and urine protein. In addition, circPAPPA2 serves as a sponge of miR-942-5p and miR-5006-3p. In conclusion, CircPAPPA2 regulates trophoblasts cell proliferation and invasion by mediating the miR-942/miR-5006-3p.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Preeclampsia / Trofoblastos / MicroARNs / ARN Circular Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: BMC Pregnancy Childbirth Asunto de la revista: OBSTETRICIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Preeclampsia / Trofoblastos / MicroARNs / ARN Circular Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: BMC Pregnancy Childbirth Asunto de la revista: OBSTETRICIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido