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Mitochondria Transplantation Promotes Corneal Epithelial Wound Healing.
Raz, Daniel; Ben-Yaakov, Keren; Levi, Michal; Bertolin, Marina; Ferrari, Stefano; Ponzin, Diego; Busin, Massimo; Leiba, Hana; Marcovich, Arie L; Eisenberg-Lerner, Avital; Rotfogel, Ziv.
Afiliación
  • Raz D; Ophthalmology Research Laboratory, Department of Ophthalmology, Kaplan Medical Center, Israel.
  • Ben-Yaakov K; Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
  • Levi M; Ophthalmology Research Laboratory, Department of Ophthalmology, Kaplan Medical Center, Israel.
  • Bertolin M; Ophthalmology Research Laboratory, Department of Ophthalmology, Kaplan Medical Center, Israel.
  • Ferrari S; Department of Ophthalmology, Kaplan Medical Center, Faculty of Medicine, Hebrew University of Jerusalem, Israel.
  • Ponzin D; Fondazione Banca degli Occhi del Veneto, Venice, Italy.
  • Busin M; Fondazione Banca degli Occhi del Veneto, Venice, Italy.
  • Leiba H; Fondazione Banca degli Occhi del Veneto, Venice, Italy.
  • Marcovich AL; Department of Translational Medicine, University of Ferrara, Ferrara, Italy.
  • Eisenberg-Lerner A; Department of Ophthalmology, Ospedali Privati Forlì "Villa Igea," Forlì, Italy.
  • Rotfogel Z; Istituto Internazionale per la Ricerca e Formazione in Oftalmologia, Forlì, Italy.
Invest Ophthalmol Vis Sci ; 65(6): 14, 2024 Jun 03.
Article en En | MEDLINE | ID: mdl-38848077
ABSTRACT

Purpose:

The integrity of the corneal epithelium is essential in maintaining normal corneal function. Conditions disrupting the corneal epithelial layer range from chemical burns to dry eye disease and may result in impairment of both corneal transparency and sensation. Identifying factors that regulate corneal wound healing is key for the development of new treatment strategies. Here, we investigated a direct role of mitochondria in corneal wound healing via mitochondria transplantation.

Methods:

Human corneal epithelial cells (hCECs) were isolated from human corneas and incubated with mitochondria which were isolated from human ARPE-19 cells. We determined the effect of mitochondria transplantation on wound healing and proliferation of hCECs. In vivo, we used a mouse model of corneal chemical injury. Mitochondria were isolated from mouse livers and topically applied to the ocular surface following injury. We evaluated the time of wound repair, corneal re-epithelization, and stromal abnormalities.

Results:

Mitochondria transplantation induced the proliferation and wound healing of primary hCECs. Further, mitochondria transplantation promoted wound healing in vivo. Specifically, mice receiving mitochondria recovered twice as fast as control mice following corneal injury, presenting both enhanced and improved repair. Corneas treated with mitochondria demonstrated the re-epithelization of the wound area to a multi-layer appearance, compared to thinning and complete loss of the epithelium in control mice. Mitochondria transplantation also prevented the thickening and disorganization of the corneal stromal lamella, restoring normal corneal dehydration.

Conclusions:

Mitochondria promote corneal re-epithelization and wound healing. Augmentation of mitochondria levels via mitochondria transplantation may serve as an effective treatment for inducing the rapid repair of corneal epithelial defects.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Epitelio Corneal / Proliferación Celular / Modelos Animales de Enfermedad / Mitocondrias Límite: Animals / Humans Idioma: En Revista: Invest Ophthalmol Vis Sci Año: 2024 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Epitelio Corneal / Proliferación Celular / Modelos Animales de Enfermedad / Mitocondrias Límite: Animals / Humans Idioma: En Revista: Invest Ophthalmol Vis Sci Año: 2024 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Estados Unidos