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Chemo- and regio-selective differential modification of native cysteines on an antibody via the use of dehydroalanine forming reagents.
Yap, Steven Y; Butcher, Tobias; Spears, Richard J; McMahon, Clíona; Thanasi, Ioanna A; Baker, James R; Chudasama, Vijay.
Afiliación
  • Yap SY; Department of Chemistry, University College London 20 Gordon Street London WC1H 0AJ UK v.chudasama@ucl.ac.uk j.r.baker@ucl.ac.uk.
  • Butcher T; Department of Chemistry, University College London 20 Gordon Street London WC1H 0AJ UK v.chudasama@ucl.ac.uk j.r.baker@ucl.ac.uk.
  • Spears RJ; Department of Chemistry, University College London 20 Gordon Street London WC1H 0AJ UK v.chudasama@ucl.ac.uk j.r.baker@ucl.ac.uk.
  • McMahon C; Department of Chemistry, University College London 20 Gordon Street London WC1H 0AJ UK v.chudasama@ucl.ac.uk j.r.baker@ucl.ac.uk.
  • Thanasi IA; Department of Chemistry, University College London 20 Gordon Street London WC1H 0AJ UK v.chudasama@ucl.ac.uk j.r.baker@ucl.ac.uk.
  • Baker JR; Department of Chemistry, University College London 20 Gordon Street London WC1H 0AJ UK v.chudasama@ucl.ac.uk j.r.baker@ucl.ac.uk.
  • Chudasama V; Department of Chemistry, University College London 20 Gordon Street London WC1H 0AJ UK v.chudasama@ucl.ac.uk j.r.baker@ucl.ac.uk.
Chem Sci ; 15(22): 8557-8568, 2024 Jun 05.
Article en En | MEDLINE | ID: mdl-38846383
ABSTRACT
Protein modification has garnered increasing interest over the past few decades and has become an important tool in many aspects of chemical biology. In recent years, much effort has focused on site-selective modification strategies that generate more homogenous bioconjugates, and this is particularly so in the antibody modification space. Modifying native antibodies by targeting solvent-accessible cysteines liberated by interchain disulfide reduction is, perhaps, the predominant strategy for achieving more site-selectivity on an antibody scaffold. This is evidenced by numerous approved antibody therapeutics that have utilised cysteine-directed conjugation reagents and the plethora of methods/strategies focused on antibody cysteine modification. However, all of these methods have a common feature in that after the reduction of native solvent-accessible cystines, the liberated cysteines are all reacted in the same manner. Herein, we report the discovery and application of dehydroalanine forming reagents (including novel reagents) capable of regio- and chemo-selectively modifying these cysteines (differentially) on a clinically relevant antibody fragment and a full antibody. We discovered that these reagents could enable differential reactivity between light chain C-terminal cysteines, heavy chain hinge region cysteines (cysteines with an adjacent proline residue, Cys-Pro), and other heavy chain internal cysteines. This differential reactivity was also showcased on small molecules and on the peptide somatostatin. The application of these dehydroalanine forming reagents was exemplified in the preparation of a dually modified antibody fragment and full antibody. Additionally, we discovered that readily available amide coupling agents can be repurposed as dehydroalanine forming reagents, which could be of interest to the broader field of chemical biology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Sci Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Sci Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido