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Effect of atractylenolide III on zearalenone-induced Snail1-mediated epithelial-mesenchymal transition in porcine intestinal epithelium.
Kim, Na Yeon; Kim, Myoung Ok; Shin, Sangsu; Kwon, Woo-Sung; Kim, Bomi; Lee, Joon Yeop; In Lee, Sang.
Afiliación
  • Kim NY; Department of Animal Science and Biotechnology, Kyungpook National University, Sangju, Gyeong-sangbuk-do, 37224, Republic of Korea.
  • Kim MO; Department of Animal Science and Biotechnology, Kyungpook National University, Sangju, Gyeong-sangbuk-do, 37224, Republic of Korea.
  • Shin S; Research Institute for Innovative Animal Science, Kyungpook National University, Sangju, Gyeongsangbuk-do, 37224, Republic of Korea.
  • Kwon WS; Department of Animal Science and Biotechnology, Kyungpook National University, Sangju, Gyeong-sangbuk-do, 37224, Republic of Korea.
  • Kim B; Research Institute for Innovative Animal Science, Kyungpook National University, Sangju, Gyeongsangbuk-do, 37224, Republic of Korea.
  • Lee JY; Department of Animal Science and Biotechnology, Kyungpook National University, Sangju, Gyeong-sangbuk-do, 37224, Republic of Korea.
  • In Lee S; Research Institute for Innovative Animal Science, Kyungpook National University, Sangju, Gyeongsangbuk-do, 37224, Republic of Korea.
J Anim Sci Biotechnol ; 15(1): 80, 2024 Jun 07.
Article en En | MEDLINE | ID: mdl-38845033
ABSTRACT

BACKGROUND:

The intestinal epithelium performs essential physiological functions, such as nutrient absorption, and acts as a barrier to prevent the entry of harmful substances. Mycotoxins are prevalent contaminants found in animal feed that exert harmful effects on the health of livestock. Zearalenone (ZEA) is produced by the Fusarium genus and induces gastrointestinal dysfunction and disrupts the health and immune system of animals. Here, we evaluated the molecular mechanisms that regulate the effects of ZEA on the porcine intestinal epithelium.

RESULTS:

Treatment of IPEC-J2 cells with ZEA decreased the expression of E-cadherin and increased the expression of Snai1 and Vimentin, which induced Snail1-mediated epithelial-to-mesenchymal transition (EMT). In addition, ZEA induces Snail-mediated EMT through the activation of TGF-ß signaling. The treatment of IPEC-J2 cells with atractylenolide III, which were exposed to ZEA, alleviated EMT.

CONCLUSIONS:

Our findings provide insights into the molecular mechanisms of ZEA toxicity in porcine intestinal epithelial cells and ways to mitigate it.
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Anim Sci Biotechnol Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Anim Sci Biotechnol Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido