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Structural properties and microbial diversity of the biofilm colonizing plastic substrates in Terra Nova Bay (Antarctica).
Papale, Maria; Fazi, Stefano; Severini, Maila; Scarinci, Roberta; Dell'Acqua, Ombretta; Azzaro, Maurizio; Venuti, Valentina; Fazio, Barbara; Fazio, Enza; Crupi, Vincenza; Irrera, Alessia; Rizzo, Carmen; Giudice, Angelina Lo; Caruso, Gabriella.
Afiliación
  • Papale M; Institute of Polar Sciences, National Research Council (CNR-ISP), Spianata S. Raineri 86, 98122 Messina, Italy.
  • Fazi S; Water Research Institute, National Research Council (CNR-IRSA), Via Salaria km 29.300 CP10, 00015 Monterotondo, Rome, Italy; National Biodiversity Future Center, Piazza Marina 61, 90133 Palermo, Italy.
  • Severini M; Water Research Institute, National Research Council (CNR-IRSA), Via Salaria km 29.300 CP10, 00015 Monterotondo, Rome, Italy.
  • Scarinci R; Water Research Institute, National Research Council (CNR-IRSA), Via Salaria km 29.300 CP10, 00015 Monterotondo, Rome, Italy.
  • Dell'Acqua O; DISTAV, Department of Earth, Environmental and Life Sciences, University of Genoa, Corso Europa, 26, 16132 Genoa, Italy.
  • Azzaro M; Institute of Polar Sciences, National Research Council (CNR-ISP), Spianata S. Raineri 86, 98122 Messina, Italy.
  • Venuti V; Department of Mathematical and Computer Sciences, Physical Sciences and Earth Sciences, University of Messina, Viale Ferdinando Stagno D'Alcontres 31, 98166 Messina, Italy.
  • Fazio B; URT "LabSens of Beyond Nano" of the Department of Physical Sciences and Technologies of Matter, National Research Council (CNR- DSFTM-ME), Viale Ferdinando Stagno D'Alcontres 31, 98166 Messina, Italy; Institute for Chemical and Physical Processes, National Research Council (CNR-IPCF), Viale Ferdinan
  • Fazio E; Department of Mathematical and Computer Sciences, Physical Sciences and Earth Sciences, University of Messina, Viale Ferdinando Stagno D'Alcontres 31, 98166 Messina, Italy.
  • Crupi V; Department of Mathematical and Computer Sciences, Physical Sciences and Earth Sciences, University of Messina, Viale Ferdinando Stagno D'Alcontres 31, 98166 Messina, Italy.
  • Irrera A; URT "LabSens of Beyond Nano" of the Department of Physical Sciences and Technologies of Matter, National Research Council (CNR- DSFTM-ME), Viale Ferdinando Stagno D'Alcontres 31, 98166 Messina, Italy. Electronic address: alessia.irrera@cnr.it.
  • Rizzo C; Institute of Polar Sciences, National Research Council (CNR-ISP), Spianata S. Raineri 86, 98122 Messina, Italy; Marine Biotechnology Department, Stazione Zoologica Anton Dohrn, Sicily Marine Centre, Villa Pace, Contrada Porticatello 29, 98167 Messina, Italy.
  • Giudice AL; Institute of Polar Sciences, National Research Council (CNR-ISP), Spianata S. Raineri 86, 98122 Messina, Italy; National Biodiversity Future Center, Piazza Marina 61, 90133 Palermo, Italy.
  • Caruso G; Institute of Polar Sciences, National Research Council (CNR-ISP), Spianata S. Raineri 86, 98122 Messina, Italy. Electronic address: gabriella.caruso@cnr.it.
Sci Total Environ ; 943: 173773, 2024 Sep 15.
Article en En | MEDLINE | ID: mdl-38844237
ABSTRACT
Microbial colonization on plastic polymers has been extensively explored, however the temporal dynamics of biofilm community in Antarctic environments are almost unknown. As a contribute to fill this knowledge gap, the structural characteristics and microbial diversity of the biofilm associated with polyvinyl chloride (PVC) and polyethylene (PE) panels submerged at 5 m of depth and collected after 3, 9 and 12 months were investigated in four coastal sites of the Ross Sea. Additional panels placed at 5 and 20 m were retrieved after 12 months. Chemical characterization was performed by FTIR-ATR and Raman (through Surface-Enhanced Raman Scattering, SERS) spectroscopy. Bacterial community composition was quantified at a single cell level by Catalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) and Confocal Laser Scanning Microscopy (CLSM); microbial diversity was assessed by 16S rRNA gene sequencing. This multidisciplinary approach has provided new insights into microbial community dynamics during biofouling process, shedding light on the biofilm diversity and temporal succession on plastic substrates in the Ross Sea. Significant differences between free-living and microbial biofilm communities were found, with a more consolidated and structured community composition on PVC compared to PE. Spectral features ascribable to tyrosine, polysaccharides, nucleic acids and lipids characterized the PVC-associated biofilms. Pseudomonadota (among Gamma-proteobacteria) and Alpha-proteobacteria dominated the microbial biofilm community. Interestingly, in Road Bay, close to the Italian "Mario Zucchelli" research station, the biofilm growth - already observed during summer season, after 3 months of submersion - continued afterwards leading to a massive microbial abundance at the end of winter (after 12 months). After 3 months, higher percentages of Gamma-proteobacteria in Road Bay than in the not-impacted site were found. These observations lead us to hypothesize that in this site microbial fouling developed during the first 3 months could serve as a starter pioneering community stimulating the successive growth during winter.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plásticos / Biopelículas / Bahías / Microbiota Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plásticos / Biopelículas / Bahías / Microbiota Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Países Bajos