Your browser doesn't support javascript.
loading
Identification of COP9 signalosome (CSN) subunits and antiviral function analysis of CSN5 in shrimp.
Lv, Li-Xia; Zhang, Qiang; Zhao, Xiao-Fan; Wang, Jin-Xing.
Afiliación
  • Lv LX; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, Shandong, China.
  • Zhang Q; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, Shandong, China.
  • Zhao XF; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, Shandong, China. Electronic address: xfzhao@sdu.edu.cn.
  • Wang JX; Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, Shandong, China. Electronic address: jxwang@sdu.edu.cn.
Fish Shellfish Immunol ; 151: 109679, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38844185
ABSTRACT
The constitutive photomorphogenesis 9 (COP9) signalosome (CSN) typically composing of eight subunits (CSN1-8) mediates the process of deneddylation and deubiquitination. The fifth subunit of COP9 signalosome, CSN5, has special characteristics compared with the other seven subunits, and plays vital roles in the deneddylation activity and diverse cellular processes. However, the role of CSN5 in antiviral immunity is not clear. In this study, we identified 8 subunits (CSN1-8) of COP9 signalosome in shrimp Marsupenaeus japonicus. CSN1-6 were existed in all tested tissues, but CSN7-CSN8 were not detected in hepatopancreas. After WSSV challenged, the expression level of Csn1 to Csn4, and Csn6 to Csn8 were highly decreased, but the expression level of Csn5 was conspicuously increased in shrimp challenged by white spot syndrome virus (WSSV). The CSN5 was recombinantly expressed in Escherichia coli and its polyclonal antibody was prepared. The expression level of CSN5 was conspicuously increased at RNA and protein levels in the shrimp challenged by WSSV. After knockdown of Csn5 by RNA interference, the WSSV replication was obviously increased in shrimp. When injected the recombinant protein of CSN5 with the membrane penetrating peptide into shrimp, WSSV replication was inhibited and the survival rate of shrimp was significantly improved compared with control. We further analyzed the expression of antimicrobial peptides (AMPs) in Csn5-RNAi shrimp, and the results showed that the expression of several AMPs was declined significantly. These results indicate that CSN5 inhibits replication of WSSV via regulating expression of AMPs in shrimp, and the recombinant CSN5 might be used in shrimp aquaculture for the white spot syndrome disease control.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Penaeidae / Virus del Síndrome de la Mancha Blanca 1 / Proteínas de Artrópodos / Complejo del Señalosoma COP9 / Inmunidad Innata Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Penaeidae / Virus del Síndrome de la Mancha Blanca 1 / Proteínas de Artrópodos / Complejo del Señalosoma COP9 / Inmunidad Innata Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido