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Low oxygen environment effect on the tomato cell wall composition during the fruit ripening process.
Leszczuk, Agata; Kutyrieva-Nowak, Nataliia; Nowak, Artur; Nosalewicz, Artur; Zdunek, Artur.
Afiliación
  • Leszczuk A; Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, Lublin, 20-290, Poland. a.leszczuk@ipan.lublin.pl.
  • Kutyrieva-Nowak N; Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, Lublin, 20-290, Poland.
  • Nowak A; Department of Industrial and Environmental Microbiology, Institute of Biological Sciences, Maria Curie- Sklodowska University, Akademicka 19, Lublin, 20-033, Poland.
  • Nosalewicz A; Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, Lublin, 20-290, Poland.
  • Zdunek A; Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, Lublin, 20-290, Poland.
BMC Plant Biol ; 24(1): 503, 2024 Jun 06.
Article en En | MEDLINE | ID: mdl-38840061
ABSTRACT

BACKGROUND:

Oxygen concentration is a key characteristic of the fruit storage environment determining shelf life and fruit quality. The aim of the work was to identify cell wall components that are related to the response to low oxygen conditions in fruit and to determine the effects of such conditions on the ripening process. Tomato (Solanum lycopersicum) fruits at different stages of the ripening process were stored in an anoxic and hypoxic environment, at 0% and 5% oxygen concentrations, respectively. We used comprehensive and comparative

methods:

from microscopic immunolabelling and estimation of enzymatic activities to detailed molecular approaches. Changes in the composition of extensin, arabinogalactan proteins, rhamnogalacturonan-I, low methyl-esterified homogalacturonan, and high methyl-esterified homogalacturonan were analysed.

RESULTS:

In-depth molecular analyses showed that low oxygen stress affected the cell wall composition, i.e. changes in protein content, a significantly modified in situ distribution of low methyl-esterified homogalacturonan, appearance of callose deposits, disturbed native activities of ß-1,3-glucanase, endo-ß-1,4-glucanase, and guaiacol peroxidase (GPX), and disruptions in molecular parameters of single cell wall components. Taken together, the data obtained indicate that less significant changes were observed in fruit in the breaker stage than in the case of the red ripe stage. The first symptoms of changes were noted after 24 h, but only after 72 h, more crucial deviations were visible. The 5% oxygen concentration slows down the ripening process and 0% oxygen accelerates the changes taking place during ripening.

CONCLUSIONS:

The observed molecular reset occurring in tomato cell walls in hypoxic and anoxic conditions seems to be a result of regulatory and protective mechanisms modulating ripening processes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Proteínas de Plantas / Pared Celular / Pectinas / Solanum lycopersicum / Frutas Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Proteínas de Plantas / Pared Celular / Pectinas / Solanum lycopersicum / Frutas Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido