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Effect of exercise duration on toluene-induced locomotor sensitization in mice: a focus on the Renin Angiotensin System.
Gallardo-Ortíz, Itzell A; Oros-González, Alain; Rodríguez-Manzo, Gabriela; Garduño-Gutiérrez, René; Aragón-Martínez, Andrés; Páez-Martínez, Nayeli.
Afiliación
  • Gallardo-Ortíz IA; Unidad de Biomedicina, Carrera de Enfermería, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Estado de México, México.
  • Oros-González A; Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, México.
  • Rodríguez-Manzo G; Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav), Sede Sur, Ciudad de México, México.
  • Garduño-Gutiérrez R; Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados del IPN (Cinvestav), Sede Sur, Ciudad de México, México.
  • Aragón-Martínez A; Unidad de Biomedicina, Carrera de Biología, Laboratorio de Gametos y Desarrollo Tecnológico, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Estado de México, México.
  • Páez-Martínez N; Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, México. npaezm@ipn.mx.
Psychopharmacology (Berl) ; 241(10): 2157-2170, 2024 Oct.
Article en En | MEDLINE | ID: mdl-38839630
ABSTRACT
RATIONALE Exercise attenuates addictive behavior; however, little is known about the contribution of exercise duration to this positive effect. The Renin Angiotensin System (RAS) has been implicated both in addictive responses and in the beneficial effects of exercise; though, its role in the advantageous effects of exercise on toluene-induced addictive responses has not been explored.

OBJECTIVES:

To evaluate the impact of different exercise regimens in mitigating the expression of toluene-induced locomotor sensitization and to analyze changes in RAS elements' expression at the mesocorticolimbic system after repeated toluene exposure and following voluntary wheel running in toluene-sensitized animals.

METHODS:

Toluene-induced addictive-like response was evaluated with a locomotor sensitization model in mice. Toluene-sensitized animals had access to running wheels 1, 2, 4 or 24 h/day for 4 weeks; thereafter, locomotor sensitization expression was evaluated after a toluene challenge. RAS elements (ACE and ACE2 enzymes; AT1, AT2 and Mas receptors) expression was determined by Western blot in the VTA, NAc and PFCx of toluene-sensitized mice with and without exercise.

RESULTS:

Individual differences in toluene-induced locomotor sensitization development were observed. Access to wheel running 1 and 2 h/day reduced but 4 and 24 h/day completely blocked locomotor sensitization expression. Repeated toluene exposure changed RAS elements' expression in the VTA, NAc and PFCx, while exercise mainly modified ACE and AT1 in air-exposed and toluene-sensitized mice.

CONCLUSIONS:

Inhalant-exposed animals show different sensitization phenotypes. Exercise duration determined its efficacy to attenuate the addictive-like response. Toluene exposure and exercise each modified RAS, the latter also modifying toluene-induced changes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Condicionamiento Físico Animal / Sistema Renina-Angiotensina / Tolueno Límite: Animals Idioma: En Revista: Psychopharmacology (Berl) Año: 2024 Tipo del documento: Article Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Condicionamiento Físico Animal / Sistema Renina-Angiotensina / Tolueno Límite: Animals Idioma: En Revista: Psychopharmacology (Berl) Año: 2024 Tipo del documento: Article Pais de publicación: Alemania