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RBM15 facilities lung adenocarcinoma cell progression by regulating RASSF8 stability through N6 Methyladenosine modification.
Ma, Mingsheng; Wang, Wei; Li, Li; Wang, Xiaoyan; Huang, Qiubo; Zhou, Chen; Huang, Yunchao; Zhao, Guangqiang; Ye, Lianhua.
Afiliación
  • Ma M; Department of Thoracic Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan,650118, China; Department of Thoracic Surgery, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan,653100, China.
  • Wang W; Department of Thoracic Surgery, Taihe Hospital (Hubei University of Medicine), Shiyan, Hubei,442000, China.
  • Li L; Department of Biotherapy Chemotherapy Center, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan,650118, China.
  • Wang X; Department of Thoracic Surgery, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan,653100, China.
  • Huang Q; Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan,650118, China.
  • Zhou C; Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan,650118, China.
  • Huang Y; Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan,650118, China.
  • Zhao G; Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan,650118, China.
  • Ye L; Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan,650118, China. Electronic address: lhye1204@aliyun.com.
Transl Oncol ; 46: 102018, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38838436
ABSTRACT
Invasion and migration are the primary factors for mortality in lung adenocarcinoma (LUAD) patients. The precise role of RNA-binding motif protein15 (RBM15)-mediated m6A modification in LUAD is not yet fully clarified. This research aims to elucidate the mechanism of RBM15 in the invasion and migration of LUAD. Western blot and dot blot assay results showed that RBM15 and methylation levels of m6A were highly expressed in LUAD tissues. Overexpression of RBM15 by lentivirus transfection increased m6A levels and promoted the invasion, migration, and proliferation of A549 and H1734 cells. Knockdown of RBM15 by lentivirus transfection had opposite effects on m6A levels, invasion, migration, and proliferation of A549 and H1734 cells. The results of nude mouse proliferation models confirmed that RBM15 knockdown inhibited in vivo tumor proliferation . Sequencing and immunoprecipitation identified RASSF8 as an interacting protein of RBM15 involved in cell invasion and migration. RBM15-mediated m6A modification inhibited RASSF8 protein levels and increased LUAD cell invasion and migration. The rescue assays demonstrated that the regulation of RBM15 on LUAD cell invasion and migration was partially rescued by RASSF8. In conclusion, RBM15-mediated m6A modification inhibits the RASSF8 protein levels and increases cell invasion and migration. Thus, targeting the RBM15-m6A-RASSF8 axis may be a promising strategy for repressing LUAD cell invasion and migration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Transl Oncol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Transl Oncol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos