LINC00520 promotes colorectal cancer progression through miRNA-195-3p / NAT2 axis.

Liu, Haibin; Zhang, Guochao; Li, Chaofeng; Song, Xin

Liu, Haibin; Zhang, Guochao; Li, Chaofeng; Song, Xin.

Afiliación

Liu H; Department of Gastrointestinal Surgery, China-Japan Friendship Hospital, Beijing, China. dr_haibinliu1971@163.com.
Zhang G; Department of Gastrointestinal Surgery, China-Japan Friendship Hospital, Beijing, China. zhgcmd@163.com.
Li C; Department of Gastrointestinal Surgery, China-Japan Friendship Hospital, Beijing, China. li-chao-feng@139.com.
Song X; Department of Gastrointestinal Surgery, China-Japan Friendship Hospital, Beijing, China. songxinxin1971@163.com.

Cell Mol Biol (Noisy-le-grand) ; 70(6): 187-191, 2024 Jun 05.

Article en En | MEDLINE | ID: mdl-38836661

ABSTRACT

ABSTRACT

In this study, we investigated the role of LINC00520 in colorectal cancer (CRC) progression. We analyzed LINC00520 expression in 15 pairs of CRC tissues and adjacent tissues using qRT-PCR, revealing significantly elevated levels in CRC tissues and cell lines. Lentivirus-mediated up/down-regulation of LINC00520 in CRC cell lines demonstrated that increased LINC00520 expression enhanced cell invasiveness, as confirmed by transwell and wound healing assays. Bioinformatics analysis identified a regulatory axis involving LINC00520, microRNA-195-3p, and NAT2. Luciferase assays confirmed direct binding between LINC00520 and microRNA-195-3p, as well as microRNA-195-3p and NAT2. Overexpression of NAT2 reversed the inhibitory effects on invasion and migration induced by LINC00520 silencing. This suggests that LINC00520, highly expressed in CRC tissues, may modulate tumor biological functions through the microRNA-195-3p/NAT2 axis. Our findings provide insights into the mechanism underlying CRC progression, highlighting the potential of LINC00520 as a therapeutic target.

Asunto(s)

Arilamina N-Acetiltransferasa; Movimiento Celular; Neoplasias Colorrectales; Progresión de la Enfermedad; Regulación Neoplásica de la Expresión Génica; MicroARNs; ARN Largo no Codificante; Humanos; MicroARNs/genética; MicroARNs/metabolismo; Neoplasias Colorrectales/genética; Neoplasias Colorrectales/patología; Neoplasias Colorrectales/metabolismo; Línea Celular Tumoral; ARN Largo no Codificante/genética; ARN Largo no Codificante/metabolismo; Arilamina N-Acetiltransferasa/genética; Arilamina N-Acetiltransferasa/metabolismo; Movimiento Celular/genética; Invasividad Neoplásica/genética; Proliferación Celular/genética

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arilamina N-Acetiltransferasa / Neoplasias Colorrectales / Regulación Neoplásica de la Expresión Génica / Movimiento Celular / Progresión de la Enfermedad / MicroARNs / ARN Largo no Codificante Límite: Humans Idioma: En Revista: Cell Mol Biol (Noisy-le-grand) Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Francia

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google