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Epitope Mapping of an Anti-Mouse CCR8 Monoclonal Antibody C8Mab-2 Using Flow Cytometry.
Kobayashi, Hiyori; Suzuki, Hiroyuki; Tanaka, Tomohiro; Kaneko, Mika K; Kato, Yukinari.
Afiliación
  • Kobayashi H; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Suzuki H; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Tanaka T; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Kaneko MK; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Kato Y; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
Monoclon Antib Immunodiagn Immunother ; 43(4): 101-107, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38836509
ABSTRACT
The C-C motif chemokine receptor 8 (CCR8) is highly and selectively expressed in regulatory T (Treg) cells and is associated with tumor progression. The massive accumulation of Treg cells into tumors suppresses the effector function of CD8+ cells against tumor cells. Therefore, selective depletion of Treg cells using anti-CCR8 monoclonal antibodies (mAbs) reinvigorates antitumor immune responses and improves responses to cancer immunotherapy. Previously, we developed an anti-mouse CCR8 (mCCR8) mAb, C8Mab-2, using the Cell-Based Immunization and Screening method. In this study, the binding epitope of C8Mab-2 was investigated using flow cytometry. The mCCR8 extracellular domain-substituted mutant analysis showed that C8Mab-2 recognizes the N-terminal region (1-33 amino acids) of mCCR8. Next, 1×alanine (or glycine) scanning and 2×alanine (or glycine) scanning were conducted in the N-terminal region. The results revealed that the 17-DFFTAP-22 sequence is important for the recognition by C8Mab-2, and Thr20 is a central amino acid of the epitope. These results revealed the involvement of the N-terminus of mCCR8 in the recognition by C8Mab-2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mapeo Epitopo / Receptores CCR8 / Citometría de Flujo / Anticuerpos Monoclonales Límite: Animals / Humans Idioma: En Revista: Monoclon Antib Immunodiagn Immunother Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mapeo Epitopo / Receptores CCR8 / Citometría de Flujo / Anticuerpos Monoclonales Límite: Animals / Humans Idioma: En Revista: Monoclon Antib Immunodiagn Immunother Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos