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Untargeted metabolomics and metagenomics reveal signatures for intramammary ceftiofur treatment and lactation stage in the cattle hindgut.
Vasco, Karla A; Hansen, Zoe A; Schilmiller, Anthony L; Bowcutt, Bailey; Carbonell, Samantha L; Ruegg, Pamela L; Quinn, Robert A; Zhang, Lixin; Manning, Shannon D.
Afiliación
  • Vasco KA; Department of Microbiology, Genetics and Immunology, Michigan State University, East Lansing, MI, United States.
  • Hansen ZA; Department of Microbiology, Genetics and Immunology, Michigan State University, East Lansing, MI, United States.
  • Schilmiller AL; Research Technology Support Facility, Mass Spectrometry and Metabolomics Core, Michigan State University, East Lansing, MI, United States.
  • Bowcutt B; Department of Microbiology, Genetics and Immunology, Michigan State University, East Lansing, MI, United States.
  • Carbonell SL; Department of Microbiology, Genetics and Immunology, Michigan State University, East Lansing, MI, United States.
  • Ruegg PL; Department of Large Animal and Clinical Sciences, Michigan State University, East Lansing, MI, United States.
  • Quinn RA; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, United States.
  • Zhang L; Department of Microbiology, Genetics and Immunology, Michigan State University, East Lansing, MI, United States.
  • Manning SD; Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, MI, United States.
Front Mol Biosci ; 11: 1364637, 2024.
Article en En | MEDLINE | ID: mdl-38836107
ABSTRACT
The gut microbiota in cattle is essential for protein, energy, and vitamin production and hence, microbiota perturbations can affect cattle performance. This study evaluated the effect of intramammary (IMM) ceftiofur treatment and lactation stage on the functional gut microbiome and metabolome. Forty dairy cows were enrolled at dry-off. Half received IMM ceftiofur and a non-antibiotic teat sealant containing bismuth subnitrate (cases), while the other half received the teat sealant (controls). Fecal samples were collected before treatment at dry off, during the dry period (weeks 1 and 5) and the first week after calving (week 9). Shotgun metagenomic sequencing was applied to predict microbial metabolic pathways whereas untargeted metabolomics was used identify polar and nonpolar metabolites. Compared to controls, long-term changes were observed in the cows given ceftiofur, including a lower abundance of microbial pathways linked to energy production, amino acid biosynthesis, and other vital molecules. The metabolome of treated cows had elevated levels of stachyose, phosphatidylethanolamine diacylglycerol (PE-DAG), and inosine a week after the IMM ceftiofur application, indicating alterations in microbial fermentation, lipid metabolism, energy, and cellular signaling. Differences were also observed by sampling, with cows in late lactation having more diverse metabolic pathways and a unique metabolome containing higher levels of histamine and histamine-producing bacteria. These data illustrate how IMM ceftiofur treatment can alter the functionality of the hindgut metabolome and microbiome. Understanding how antibiotics and lactation stages, which are each characterized by unique diets and physiology, impact the function of resident microbes is critical to define normal gut function in dairy cattle.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Mol Biosci Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Mol Biosci Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza