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Characterisation of circulating tumor-associated and immune cells in patients with advanced-stage non-small cell lung cancer.
Yaghoubi Naei, Vahid; Ivanova, Ekaterina; Mullally, William; O'Leary, Connor G; Ladwa, Rahul; O'Byrne, Ken; Warkiani, Majid E; Kulasinghe, Arutha.
Afiliación
  • Yaghoubi Naei V; School of Biomedical Engineering University of Technology Sydney Sydney NSW Australia.
  • Ivanova E; Frazer Institute, Faculty of Medicine The University of Queensland Brisbane QLD Australia.
  • Mullally W; Cancer and Ageing Research Program, Centre for Genomics and Personalised Health Queensland University of Technology Woolloongabba QLD Australia.
  • O'Leary CG; The Princess Alexandra Hospital Brisbane QLD Australia.
  • Ladwa R; The Princess Alexandra Hospital Brisbane QLD Australia.
  • O'Byrne K; Frazer Institute, Faculty of Medicine The University of Queensland Brisbane QLD Australia.
  • Warkiani ME; The Princess Alexandra Hospital Brisbane QLD Australia.
  • Kulasinghe A; The Princess Alexandra Hospital Brisbane QLD Australia.
Clin Transl Immunology ; 13(6): e1516, 2024.
Article en En | MEDLINE | ID: mdl-38835954
ABSTRACT

Objectives:

Globally, non-small cell lung cancer (NSCLC) is the most prevalent form of lung cancer and the leading cause of cancer-related deaths. Tumor-associated circulating cells in NSCLC can have a wide variety of morphological and phenotypic characteristics, including epithelial, immunological or hybrid subtypes. The distinctive characteristics and potential clinical significance of these cells in patients with NSCLC are explored in this study.

Methods:

We utilised a spiral microfluidic device to enrich large cells and cell aggregates from the peripheral blood samples of NSCLC patients. These cells were characterised through high-resolution immunofluorescent imaging and statistical analysis, correlating findings with clinical information from our patient cohort.

Results:

We have identified varied populations of heterotypic circulating tumor cell clusters with differing immune cell composition that included a distinct class of atypical tumor-associated macrophages that exhibits unique morphology and cell size. This subtype's prevalence is positively correlated with the tumor stage, progression and metastasis.

Conclusions:

Our study reveals a heterogeneous landscape of circulating tumor cells and their clusters, underscoring the complexity of NSCLC pathobiology. The identification of a unique subtype of atypical tumor-associatedmacrophages that simultaneously express both tumor and immune markers and whose presence correlates with late disease stages, poor clinical outcomes and metastatic risk infers  the potential of these cells as biomarkers for NSCLC staging and prognosis. Future studies should focus on the role of these cells in the tumor microenvironment and their potential as therapeutic targets. Additionally, longitudinal studies tracking these cell types through disease progression could provide further insights into their roles in NSCLC evolution and response to treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Clin Transl Immunology Año: 2024 Tipo del documento: Article Pais de publicación: Australia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Clin Transl Immunology Año: 2024 Tipo del documento: Article Pais de publicación: Australia