Mapping-based localization of blackleg-resistant candidate genes of Chinese cabbage (Brassica rapa).
Plant Dis
; 2024 Jun 03.
Article
en En
| MEDLINE
| ID: mdl-38831591
ABSTRACT
Recently, blackleg disease has seriously impacted the cultivation and development of Brassica crops. In this study, we conducted mapping-based localization of blackleg-resistant candidate genes in Chinese cabbage. Through phenotype evaluation, Chinese cabbage materials 15S414 and 15S420 were selected as blackleg-resistant and blackleg-susceptible parents, respectively. Inheritance pattern analysis suggested that the dominant major genes mainly determined the blackleg resistance of Chinese cabbage. Upon bulked segregation analysis , the blackleg-resistant candidate genes were initially located within a 4.3 Mb interval on chromosome A06. Through construction of the genetic linkage map, blackleg-resistant candidate genes were further limited to a region of 160 kb containing seven resistance-related genes. Coding sequence variation analysis revealed that all seven resistance-related genes displayed various degrees of single nucleotide polymorphism variations between parent materials 15S414 and 15S420.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Plant Dis
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Estados Unidos