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Screening and functional verification of drought resistance-related genes in castor bean seeds.
Wang, Zhiyan; Luo, Rui; Wen, Qi; Liang, Xiaotian; Zhao, Huibo; Zhao, Yong; Yin, Mingda; Wen, Yanpeng; Hu, Xuemei; Huang, Fenglan.
Afiliación
  • Wang Z; College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia, China.
  • Luo R; College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia, China.
  • Wen Q; College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia, China.
  • Liang X; Agronomy College, Jilin Agricultural University, Changchun, 130118, China.
  • Zhao H; National Oat Improvement Center, Baicheng Academy of Agricultural Sciences, Baicheng, 137000, China.
  • Zhao Y; China National Rice Research Institute, Hangzhou, 311400, China.
  • Yin M; School of Life Sciences, Laboratory of Genetics, Baicheng Normal University, Baicheng, 137000, China.
  • Wen Y; College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia, China.
  • Hu X; College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia, China.
  • Huang F; College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao, 028000, Inner Mongolia, China.
BMC Plant Biol ; 24(1): 493, 2024 Jun 03.
Article en En | MEDLINE | ID: mdl-38831288
ABSTRACT
Drought is one of the natural stresses that greatly impact plants. Castor bean (Ricinus communis L.) is an oil crop with high economic value. Drought is one of the factors limiting castor bean growth. The drought resistance mechanisms of castor bean have become a research focus. In this study, we used castor germinating embryos as experimental materials, and screened genes related to drought resistance through physiological measurements, proteomics and metabolomics joint analysis; castor drought-related genes were subjected to transient silencing expression analysis in castor leaves to validate their drought-resistant functions, and heterologous overexpression and backward complementary expression in Arabidopsis thaliana, and analysed the mechanism of the genes' response to the participation of Arabidopsis thaliana in drought-resistance.Three drought tolerance-related genes, RcECP 63, RcDDX 31 and RcA/HD1, were obtained by screening and analysis, and transient silencing of expression in castor leaves further verified that these three genes corresponded to drought stress, and heterologous overexpression and back-complementary expression of the three genes in Arabidopsis thaliana revealed that the function of these three genes in drought stress response.In this study, three drought tolerance related genes, RcECP 63, RcDDX 31 and RcA/HD1, were screened and analysed for gene function, which were found to be responsive to drought stress and to function in drought stress, laying the foundation for the study of drought tolerance mechanism in castor bean.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semillas / Ricinus communis / Arabidopsis / Sequías Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semillas / Ricinus communis / Arabidopsis / Sequías Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido