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Photoelectrochemical immunoassay of cardiac troponin I based on ZnTCPP/CdIn2S4 type-II heterojunction and co-catalyzed precipitation biolabeling.
Li, Jiahui; Tang, Fengci; Xie, Qingji; Zeng, Xingyu; He, Fang; Xie, Qingji.
Afiliación
  • Li J; Hunan Normal University, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, 410081, Changsha, China.
  • Tang F; Hunan Normal University, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, 410081, Changsha, China.
  • Xie Q; Hunan Normal University, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, 410081, Changsha, China. xieqj@hunnu.edu.cn.
  • Zeng X; Hunan Normal University, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, 410081, Changsha, China.
  • He F; Hunan Normal University, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, 410081, Changsha, China.
  • Xie Q; Hunan Normal University, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, 410081, Changsha, China.
Mikrochim Acta ; 191(7): 364, 2024 06 03.
Article en En | MEDLINE | ID: mdl-38831034
ABSTRACT
CdIn2S4 and zinc tetrakis(4-carboxyphenyl)porphyrin (ZnTCPP) were synthesized by hydrothermal method, and an organic dye-sensitized inorganic semiconductor ZnTCPP/CdIn2S4 type II heterojunction was constructed on a fluorine-doped tin oxide (FTO) substrate electrode. A sandwich immunostructure for signal-attenuation photoelectrochemical (PEC) detection of cardiac troponin I (cTnI) was constructed using the ZnTCPP/CdIn2S4/FTO photoanode and a horseradish peroxidase (HRP)-ZnFe2O4-Ab2-bovine serum albumin (BSA) immunolabeling complex. The bioenzyme HRP and the HRP-like nanozyme ZnFe2O4 can co-catalyze the oxidation of 4-chloro-1-naphthol (4-CN) by H2O2 to produce an insoluble precipitate on the photoanode, thus notably reducing the anodic photocurrent for quantitative determination of cTnI. Under the optimal conditions, the photocurrent at 0 V vs. SCE in 0.1 M phosphate buffer solution (pH 7.40) containing 0.1 M ascorbic acid was linear with the logarithm of cTnI concentration from 500 fg mL-1 to 50.0 ng mL-1, and the limit of detection (LOD, S/N = 3) is 0.15 pg mL-1. Spiked recoveries were 95.1% ~ 104% for assay of cTnI in human serum samples.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Estaño / Troponina I / Técnicas Electroquímicas / Límite de Detección Límite: Animals / Humans Idioma: En Revista: Mikrochim Acta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Austria
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Estaño / Troponina I / Técnicas Electroquímicas / Límite de Detección Límite: Animals / Humans Idioma: En Revista: Mikrochim Acta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Austria