Insertion sequence transposition activates antimycobacteriophage immunity through an <i>lsr2</i>-silenced lipid metabolism gene island.

Li, Yakun; Wei, Yuyun; Guo, Xiao; Li, Xiaohui; Lu, Lining; Hu, Lihua; He, Zheng-Guo

Insertion sequence transposition activates antimycobacteriophage immunity through an lsr2-silenced lipid metabolism gene island.

Li, Yakun; Wei, Yuyun; Guo, Xiao; Li, Xiaohui; Lu, Lining; Hu, Lihua; He, Zheng-Guo.

Afiliación

Li Y; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology Guangxi University Nanning China.
Wei Y; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology Guangxi University Nanning China.
Guo X; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology Guangxi University Nanning China.
Li X; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology Guangxi University Nanning China.
Lu L; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology Guangxi University Nanning China.
Hu L; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology Guangxi University Nanning China.
He ZG; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology Guangxi University Nanning China.

mLife ; 3(1): 87-100, 2024 Mar.

Article en En | MEDLINE | ID: mdl-38827510

ABSTRACT

ABSTRACT

Insertion sequences (ISs) exist widely in bacterial genomes, but their roles in the evolution of bacterial antiphage defense remain to be clarified. Here, we report that, under the pressure of phage infection, the IS1096 transposition of Mycobacterium smegmatis into the lsr2 gene can occur at high frequencies, which endows the mutant mycobacterium with a broad-spectrum antiphage ability. Lsr2 functions as a negative regulator and directly silences expression of a gene island composed of 11 lipid metabolism-related genes. The complete or partial loss of the gene island leads to a significant decrease of bacteriophage adsorption to the mycobacterium, thus defending against phage infection. Strikingly, a phage that has evolved mutations in two tail-filament genes can re-escape from the lsr2 inactivation-triggered host defense. This study uncovered a new signaling pathway for activating antimycobacteriophage immunity by IS transposition and provided insight into the natural evolution of bacterial antiphage defense.

Palabras clave

antiphage defense; bacteriophage; gene island; insertion sequence; mycobacteria

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: MLife Año: 2024 Tipo del documento: Article Pais de publicación: Australia