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Optical projection tomography implemented for accessibility and low cost (OPTImAL).
Darling, C; Kumar, S; Alexandrov, Y; de Faye, J; Almagro Santiago, J; Rýdlová, A; Bugeon, L; Dallman, M J; Behrens, A J; French, P M W; McGinty, J.
Afiliación
  • Darling C; Physics Department, Imperial College London , London SW7 2AZ, UK.
  • Kumar S; Physics Department, Imperial College London , London SW7 2AZ, UK.
  • Alexandrov Y; Francis Crick Institute , London NW1 1AT, UK.
  • de Faye J; Physics Department, Imperial College London , London SW7 2AZ, UK.
  • Almagro Santiago J; Francis Crick Institute , London NW1 1AT, UK.
  • Rýdlová A; Cancer Stem Cell Laboratory, Institute of Cancer Research , London SW7 3RP, UK.
  • Bugeon L; Cancer Stem Cell Laboratory, Institute of Cancer Research , London SW7 3RP, UK.
  • Dallman MJ; Department of Life Sciences, Imperial College London , London SW7 2AZ, UK.
  • Behrens AJ; Department of Life Sciences, Imperial College London , London SW7 2AZ, UK.
  • French PMW; Department of Life Sciences, Imperial College London , London SW7 2AZ, UK.
  • McGinty J; Cancer Stem Cell Laboratory, Institute of Cancer Research , London SW7 3RP, UK.
Philos Trans A Math Phys Eng Sci ; 382(2274): 20230101, 2024 Jul 09.
Article en En | MEDLINE | ID: mdl-38826047
ABSTRACT
Optical projection tomography (OPT) is a three-dimensional mesoscopic imaging modality that can use absorption or fluorescence contrast, and is widely applied to fixed and live samples in the mm-cm scale. For fluorescence OPT, we present OPT implemented for accessibility and low cost, an open-source research-grade implementation of modular OPT hardware and software that has been designed to be widely accessible by using low-cost components, including light-emitting diode (LED) excitation and cooled complementary metal-oxide-semiconductor (CMOS) cameras. Both the hardware and software are modular and flexible in their implementation, enabling rapid switching between sample size scales and supporting compressive sensing to reconstruct images from undersampled sparse OPT data, e.g. to facilitate rapid imaging with low photobleaching/phototoxicity. We also explore a simple implementation of focal scanning OPT to achieve higher resolution, which entails the use of a fan-beam geometry reconstruction method to account for variation in magnification. This article is part of the Theo Murphy meeting issue 'Open, reproducible hardware for microscopy'.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Philos Trans A Math Phys Eng Sci Asunto de la revista: BIOFISICA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Philos Trans A Math Phys Eng Sci Asunto de la revista: BIOFISICA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido