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Electrochemical Monitoring of Real-Time Vesicle Dynamics Induced by Tau in a Confined Nanopipette.
Chen, Ke-Le; Yu, Ru-Jia; Zhong, Cheng-Bing; Wang, Ziyi; Xie, Bao-Kang; Ma, Hui; Ao, Mingjun; Zheng, Peng; Ewing, Andrew G; Long, Yi-Tao.
Afiliación
  • Chen KL; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails.
  • Yu RJ; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails.
  • Zhong CB; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails.
  • Wang Z; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China.
  • Xie BK; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails.
  • Ma H; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails.
  • Ao M; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China.
  • Zheng P; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China.
  • Ewing AG; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, 41296, Sweden.
  • Long YT; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China E-mails.
Angew Chem Int Ed Engl ; 63(39): e202406677, 2024 Sep 23.
Article en En | MEDLINE | ID: mdl-38825572
ABSTRACT
The microtubule-associated protein tau participates in neurotransmission regulation via its interaction with synaptic vesicles (SVs). The precise nature and mechanics of tau's engagement with SVs, especially regarding alterations in vesicle dynamics, remain a matter of discussion. We report an electrochemical method using a synapse-mimicking nanopipette to monitor vesicle dynamics induced by tau. A model vesicle of ~30 nm is confined within a lipid-modified nanopipette orifice with a comparable diameter to mimic the synaptic lipid environment. Both tau and phosphorylated tau (p-tau) present two-state dynamic behavior in this biomimetic system, showing typical ionic current oscillation, induced by lipid-tau interaction. The results indicate that p-tau has a stronger affinity to the lipid vesicles in the confined environment, blocking the vesicle movement to a higher degree. Taken together, this method bridges a gap for sensing synaptic vesicle dynamics in a confined lipid environment, mimicking vesicle movement near the synaptic membrane. These findings contribute to understanding how different types of tau protein regulate synaptic vesicle motility and to underlying its functional and pathological behaviours in disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vesículas Sinápticas / Proteínas tau / Técnicas Electroquímicas Límite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2024 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vesículas Sinápticas / Proteínas tau / Técnicas Electroquímicas Límite: Humans Idioma: En Revista: Angew Chem Int Ed Engl Año: 2024 Tipo del documento: Article Pais de publicación: Alemania