Your browser doesn't support javascript.
loading
Standardizing a method for functional assessment of neural networks in brain organoids.
Oliva, M K; Bourke, J; Kornienko, D; Mattei, C; Mao, M; Kuanyshbek, A; Ovchinnikov, D; Bryson, A; Karle, T J; Maljevic, S; Petrou, S.
Afiliación
  • Oliva MK; Ion Channels and Diseases Group, The Florey, The University of Melbourne, Parkville, VIC 3052, Australia. Electronic address: megan.oliva@florey.edu.au.
  • Bourke J; Ion Channels and Diseases Group, The Florey, The University of Melbourne, Parkville, VIC 3052, Australia.
  • Kornienko D; Ion Channels and Diseases Group, The Florey, The University of Melbourne, Parkville, VIC 3052, Australia.
  • Mattei C; Ion Channels and Diseases Group, The Florey, The University of Melbourne, Parkville, VIC 3052, Australia.
  • Mao M; Ion Channels and Diseases Group, The Florey, The University of Melbourne, Parkville, VIC 3052, Australia.
  • Kuanyshbek A; Ion Channels and Diseases Group, The Florey, The University of Melbourne, Parkville, VIC 3052, Australia.
  • Ovchinnikov D; Ion Channels and Diseases Group, The Florey, The University of Melbourne, Parkville, VIC 3052, Australia.
  • Bryson A; Ion Channels and Diseases Group, The Florey, The University of Melbourne, Parkville, VIC 3052, Australia.
  • Karle TJ; Ion Channels and Diseases Group, The Florey, The University of Melbourne, Parkville, VIC 3052, Australia.
  • Maljevic S; Ion Channels and Diseases Group, The Florey, The University of Melbourne, Parkville, VIC 3052, Australia.
  • Petrou S; Ion Channels and Diseases Group, The Florey, The University of Melbourne, Parkville, VIC 3052, Australia; Praxis Precision Medicines, Inc., Cambridge, MA 02142, USA.
J Neurosci Methods ; 409: 110178, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38825241
ABSTRACT
During the last decade brain organoids have emerged as an attractive model system, allowing stem cells to be differentiated into complex 3D models, recapitulating many aspects of human brain development. Whilst many studies have analysed anatomical and cytoarchitectural characteristics of organoids, their functional characterisation has been limited, and highly variable between studies. Standardised, consistent methods for recording functional activity are critical to providing a functional understanding of neuronal networks at the synaptic and network level that can yield useful information about functional network phenotypes in disease and healthy states. In this study we outline a detailed methodology for calcium imaging and Multi-Electrode Array (MEA) recordings in brain organoids. To illustrate the utility of these functional interrogation techniques in uncovering induced differences in neural network activity we applied various stimulating media protocols. We demonstrate overlapping information from the two modalities, with comparable numbers of active cells in the four treatment groups and an increase in synchronous behaviour in BrainPhys treated groups. Further development of analysis pipelines to reveal network level changes in brain organoids will enrich our understanding of network formation and perturbation in these structures, and aid in the future development of drugs that target neurological disorders at the network level.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Organoides / Calcio / Red Nerviosa Límite: Humans Idioma: En Revista: J Neurosci Methods Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Organoides / Calcio / Red Nerviosa Límite: Humans Idioma: En Revista: J Neurosci Methods Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos