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The interplay of homology-directed repair pathways in the repair of zebularine-induced DNA-protein crosslinks in Arabidopsis.
Dvorák Tomastíková, Eva; Vaculíková, Jitka; Stenclová, Veronika; Kaduchová, Katerina; Poborilová, Zuzana; Palecek, Jan J; Pecinka, Ales.
Afiliación
  • Dvorák Tomastíková E; Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany of the Czech Academy of Sciences, Slechtitelu 31, Olomouc, 77900, Czech Republic.
  • Vaculíková J; Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany of the Czech Academy of Sciences, Slechtitelu 31, Olomouc, 77900, Czech Republic.
  • Stenclová V; Faculty of Science, National Center for Biomolecular Research, Masaryk University, Kamenice 5, Brno, 62500, Czech Republic.
  • Kaduchová K; Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany of the Czech Academy of Sciences, Slechtitelu 31, Olomouc, 77900, Czech Republic.
  • Poborilová Z; Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany of the Czech Academy of Sciences, Slechtitelu 31, Olomouc, 77900, Czech Republic.
  • Palecek JJ; Centre of Plant Structural and Functional Genomics, Institute of Experimental Botany of the Czech Academy of Sciences, Slechtitelu 31, Olomouc, 77900, Czech Republic.
  • Pecinka A; Faculty of Science, National Center for Biomolecular Research, Masaryk University, Kamenice 5, Brno, 62500, Czech Republic.
Plant J ; 119(3): 1418-1432, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38824612
ABSTRACT
DNA-protein crosslinks (DPCs) are highly toxic DNA lesions represented by proteins covalently bound to the DNA. Persisting DPCs interfere with fundamental genetic processes such as DNA replication and transcription. Cytidine analog zebularine (ZEB) has been shown to crosslink DNA METHYLTRANSFERASE1 (MET1). Recently, we uncovered a critical role of the SMC5/6-mediated SUMOylation in the repair of DPCs. In an ongoing genetic screen, we identified two additional candidates, HYPERSENSITIVE TO ZEBULARINE 2 and 3, that were mapped to REGULATOR OF TELOMERE ELONGATION 1 (RTEL1) and polymerase TEBICHI (TEB), respectively. By monitoring the growth of hze2 and hze3 plants in response to zebularine, we show the importance of homologous recombination (HR) factor RTEL1 and microhomology-mediated end-joining (MMEJ) polymerase TEB in the repair of MET1-DPCs. Moreover, genetic interaction and sensitivity assays showed the interdependency of SMC5/6 complex, HR, and MMEJ in the homology-directed repair of MET1-DPCs in Arabidopsis. Altogether, we provide evidence that MET1-DPC repair in plants is more complex than originally expected.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Citidina / Proteínas de Arabidopsis Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Citidina / Proteínas de Arabidopsis Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Reino Unido