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Exploring Asphodelus microcarpus as a source of xanthine oxidase inhibitors: Insights from in silico and in vitro studies.
Di Petrillo, Amalia; Siguri, Chiara; Delogu, Giovanna L; Fais, Antonella; Era, Benedetta; Floris, Sonia; Pintus, Francesca; Kumar, Amit; Fantini, Massimo Claudio; Olla, Stefania.
Afiliación
  • Di Petrillo A; Department of Medical Sciences and Public Health, University of Cagliari, 09042, Monserrato, Italy. Electronic address: amalia.dip@unica.it.
  • Siguri C; Institute for Genetic and Biomedical Research (IRGB), The National Research Council (CNR), 09042, Monserrato, Italy.
  • Delogu GL; Department of Life and Environmental Sciences, University of Cagliari, 09042, Monserrato, Italy.
  • Fais A; Department of Life and Environmental Sciences, University of Cagliari, 09042, Monserrato, Italy.
  • Era B; Department of Life and Environmental Sciences, University of Cagliari, 09042, Monserrato, Italy.
  • Floris S; Department of Life and Environmental Sciences, University of Cagliari, 09042, Monserrato, Italy.
  • Pintus F; Department of Life and Environmental Sciences, University of Cagliari, 09042, Monserrato, Italy.
  • Kumar A; Department of Electrical and Electronic Engineering, University of Cagliari, Via Marengo 2, 09123, Cagliari, Italy.
  • Fantini MC; Department of Medical Sciences and Public Health, University of Cagliari, 09042, Monserrato, Italy.
  • Olla S; Institute for Genetic and Biomedical Research (IRGB), The National Research Council (CNR), 09042, Monserrato, Italy.
Chem Biol Interact ; 397: 111087, 2024 Jul 01.
Article en En | MEDLINE | ID: mdl-38823536
ABSTRACT
Xanthine oxidase (XO) plays a critical role in purine catabolism, catalyzing the conversion of hypoxanthine to xanthine and xanthine to uric acid, contributing to superoxide anion production. This process is implicated in various human diseases, particularly gout. Traditional XO inhibitors, such as allopurinol and febuxostat, while effective, may present side effects. Our study focuses on Asphodelus microcarpus, a plant renowned for traditional anti-inflammatory uses. Recent investigations into its phenolic-rich flowers, notably abundant in luteolin derivatives, reveal its potential as a natural source of XO inhibitors. In the present research, XO inhibition by an ethanolic flowers extract from A. microcarpus is reported. In silico docking studies have highlighted luteolin derivatives as potential XO inhibitors, and molecular dynamics support that luteolin 7-O-glucoside has the highest binding stability compared to other compounds and controls. In vitro studies confirm that luteolin 7-O-glucoside inhibits XO more effectively than the standard inhibitor allopurinol, with an IC50 value of 4.8 µg/mL compared to 11.5 µg/mL, respectively. These findings underscore the potential therapeutic significance of A. microcarpus in managing conditions related to XO activity. The research contributes valuable insights into the health-promoting properties of A. microcarpus and its potential application in natural medicine, presenting a promising avenue for further exploration in disease management.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Xantina Oxidasa / Luteolina / Inhibidores Enzimáticos / Simulación del Acoplamiento Molecular Límite: Humans Idioma: En Revista: Chem Biol Interact Año: 2024 Tipo del documento: Article Pais de publicación: Irlanda
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Xantina Oxidasa / Luteolina / Inhibidores Enzimáticos / Simulación del Acoplamiento Molecular Límite: Humans Idioma: En Revista: Chem Biol Interact Año: 2024 Tipo del documento: Article Pais de publicación: Irlanda