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Cell-size-dependent regulation of Ezrin dictates epithelial resilience to stretch by countering myosin-II-mediated contractility.
Chouhan, Geetika; Lewis, Natasha Steffi; Ghanekar, Vallari; Koti Ainavarapu, Sri Rama; Inamdar, Mandar M; Sonawane, Mahendra.
Afiliación
  • Chouhan G; Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, India.
  • Lewis NS; Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, India.
  • Ghanekar V; Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, India.
  • Koti Ainavarapu SR; Department of Chemical Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, India.
  • Inamdar MM; Department of Civil Engineering, Indian Institute of Technology Bombay, Mumbai, India. Electronic address: minamdar@iitb.ac.in.
  • Sonawane M; Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, India. Electronic address: mahendras@tifr.res.in.
Cell Rep ; 43(6): 114271, 2024 Jun 25.
Article en En | MEDLINE | ID: mdl-38823013
ABSTRACT
The epithelial adaptations to mechanical stress are facilitated by molecular and tissue-scale changes that include the strengthening of junctions, cytoskeletal reorganization, and cell-proliferation-mediated changes in tissue rheology. However, the role of cell size in controlling these properties remains underexplored. Our experiments in the zebrafish embryonic epidermis, guided by theoretical estimations, reveal a link between epithelial mechanics and cell size, demonstrating that an increase in cell size compromises the tissue fracture strength and compliance. We show that an increase in E-cadherin levels in the proliferation-deficient epidermis restores epidermal compliance but not the fracture strength, which is largely regulated by Ezrin-an apical membrane-cytoskeleton crosslinker. We show that Ezrin fortifies the epithelium in a cell-size-dependent manner by countering non-muscle myosin-II-mediated contractility. This work uncovers the importance of cell size maintenance in regulating the mechanical properties of the epithelium and fostering protection against future mechanical stresses.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Miosina Tipo II / Proteínas del Citoesqueleto / Tamaño de la Célula Límite: Animals Idioma: En Revista: Cell Rep Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Miosina Tipo II / Proteínas del Citoesqueleto / Tamaño de la Célula Límite: Animals Idioma: En Revista: Cell Rep Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos