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Injectable thermo-responsive Poloxamer hydrogel/methacrylate gelatin microgels stimulates bone regeneration through biomimetic programmed release of SDF-1a and IGF-1.
Zhong, Qiang; Wang, Ding; Mai, Huaming; Chen, Rong; Xu, Yixin; Lei, Mingyuan; Xie, Jiajun; Tang, Zinan; Fu, Jinlang; Chen, Yuhang; Wang, Jian; Shi, Zhanjun; Cheng, Hao.
Afiliación
  • Zhong Q; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China.
  • Wang D; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China.
  • Mai H; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China.
  • Chen R; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China.
  • Xu Y; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China.
  • Lei M; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China.
  • Xie J; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China.
  • Tang Z; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China.
  • Fu J; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China.
  • Chen Y; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China.
  • Wang J; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China. Electronic address: wangjiannanfanghospital@gmail.com.
  • Shi Z; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China. Electronic address: shizhanjun111@gmail.com.
  • Cheng H; Department of Orthopedics, Nanfang Hospital, Southern Medical University, 1838 # Guangzhou North Avenue, Guangzhou, Guangdong Province 510515, People's Republic of China. Electronic address: haocool317@gmail.com.
Int J Biol Macromol ; 271(Pt 2): 132742, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38821297
ABSTRACT
Injectable hydrogels, offering adaptable drug delivery of growth factors (GFs), hold promise for treating bone defects. To optimize osteogenic efficacy, the release of GFs should mirror the natural bone healing. We developed an injectable thermo-responsive hydrogel/microgels platform for dual GF delivery for bone regeneration. Stromal cell-derived factor-1 alpha (SDF-1a) and the Methacrylate Gelatin (GelMA) microgels which encapsulated insulin-like growth factor-1 (IGF-1) loaded liposomes (Ls) were introduced into Poloxamer 407 (P407) hydrogel matrix. This system achieved the biomimetic release profile of SDF-1a and IGF-1, which covered the early stage from day 1 to 7 and the continuous stage from day 5 to 21, respectively. In vitro study confirmed the enhanced migration, osteogenic biomarker expression, and matrix mineralization of the bone marrow mesenchymal stem cells (BMSCs) co-cultivated with the dual GFs delivering hydrogel/microgels. Transcriptome sequencing revealed that the potential mechanism was associated with mitogen-activated protein kinase (MAPK) signaling activation and its downstream ribosomal protein S6 kinase 2 (RSK2) upregulation. In a critical-sized calvarial defect model in Sprague-Dawley (SD) rats, the injectable hydrogel/microgels system promoted significant bone regeneration. Collectively, our study suggested the current hydrogel/microgels system with the biomimetic release of SDF-1a and IGF-1 efficiently promoted bone regeneration, informing the future development of GF delivery systems intended for bone regeneration therapies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración Ósea / Factor I del Crecimiento Similar a la Insulina / Poloxámero / Hidrogeles / Quimiocina CXCL12 / Gelatina Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración Ósea / Factor I del Crecimiento Similar a la Insulina / Poloxámero / Hidrogeles / Quimiocina CXCL12 / Gelatina Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos