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The screening method for 39 phytotoxins and mycotoxins in blood and urine with liquid chromatography-high resolution mass spectrometry.
Yin, Yuqi; Sun, Weiyang; Wang, Xuan; Chen, Jiayue; Zeng, Hongyan; Hao, Sifan; Ren, Lin; Yong, Li; Luo, Chunying; Zou, Xiaoli.
Afiliación
  • Yin Y; Department of Public Health Laboratory Science, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
  • Sun W; Department of Public Health Laboratory Science, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
  • Wang X; Department of Public Health Laboratory Science, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
  • Chen J; Department of Public Health Laboratory Science, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
  • Zeng H; Department of Public Health Laboratory Science, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
  • Hao S; Department of Public Health Laboratory Science, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
  • Ren L; Sichuan Centre for Disease Control and Prevention, Chengdu 610041, People's Republic of China.
  • Yong L; Sichuan Centre for Disease Control and Prevention, Chengdu 610041, People's Republic of China. Electronic address: 79145020@qq.com.
  • Luo C; Chengdu Centre for Disease Control and Prevention, Chengdu 610047, People's Republic of China. Electronic address: cyluo2012@163.com.
  • Zou X; Department of Public Health Laboratory Science, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, People's Republic of China; Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, West China School of Public Health and Wes
J Chromatogr B Analyt Technol Biomed Life Sci ; 1241: 124173, 2024 Jul 01.
Article en En | MEDLINE | ID: mdl-38821004
ABSTRACT

BACKGROUND:

Poisonings caused by plant toxins and mycotoxins occur frequently, which do great harm to human health and social public health safety. When a poisoning incident occurs, biological samples are commonly be used to conduct the detection of toxic substances and their metabolites for targeted clinical treatment and incident analysis.

OBJECTIVE:

To establish an efficient and accurate analysis method of 39 phytotoxins and mycotoxins in blood and urine by high performance liquid chromatography quadrupole tandem orbitrap mass spectrometry (HPLC-Orbitrap MS).

METHOD:

After 3 mL of methanol being added to 1 mL blood and urine respectively for extraction and protein precipitation, the supernatant was injected into HPLC-Orbitrap MS for analysis. The phytotoxins and mycotoxins were separated by Hypersil GOLD PFP column with gradient elution using methanol-5 mmol/L ammonium acetate as mobile phase. The data were collected in ESI positive ion mode using Full MS/dd-MS2 for mass spectrometry detection.

RESULT:

The mass database of 39 phytotoxins and mycotoxins was developed, and accurate qualitative analysis can be obtained by matching with the database using the proposed identification criteria. Limit of detections (LODs) were 1.34 × 10-4 âˆ¼ 1.92 ng/mL and 1.92 × 10-4 âˆ¼ 9.80 ng/mL for blood and urine samples, respectively. Limits of quantification (LOQ) of toxins in blood and urine ranged from 4.47 × 10-4 âˆ¼ 6.32 ng/mL and 6.39 × 10-4 âˆ¼ 32.67 ng/mL, respectively. Intra-day relative standard deviations (RSDs) were 0.79 % âˆ¼ 10.90 %, and inter-day RSDs were 1.08 % âˆ¼ 18.93 %. The recoveries can reach 90 % âˆ¼ 110 % with matrix matching calibration curves.

CONCLUSION:

The established method is simple and rapid to operate, which can complete the sample analysis within 30 min, providing technical support for clinical poisoning treatment and public health poisoning analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Límite de Detección / Micotoxinas Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Límite de Detección / Micotoxinas Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos