Your browser doesn't support javascript.
loading
Unraveling the role of microRNAs: potential biomarkers for gestational diabetes mellitus revealed through RNA sequencing analysis.
Lin, Huizhen; Chen, Xiao; Wang, Lisui; Zhu, Tang; Feng, Xiaohui; Liu, Xiaomei; Chen, Haiying; Pan, Si.
Afiliación
  • Lin H; Department of Clinical Laboratory, The First Hospital of Putian, Teaching Hospital, Fujian Medical University, Putian, 351100, China.
  • Chen X; Department of Clinical Laboratory, The First Hospital of Putian, Teaching Hospital, Fujian Medical University, Putian, 351100, China.
  • Wang L; Department of Clinical Laboratory, The First Hospital of Putian, Teaching Hospital, Fujian Medical University, Putian, 351100, China.
  • Zhu T; Key Laboratory of Translational Tumor Medicine in Fujian Province, Putian University, School of Basic Medicine Science, Putian, 351100, Fujian, China.
  • Feng X; Department of Clinical Laboratory, The First Hospital of Putian, Teaching Hospital, Fujian Medical University, Putian, 351100, China.
  • Liu X; Department of Clinical Laboratory, The First Hospital of Putian, Teaching Hospital, Fujian Medical University, Putian, 351100, China.
  • Chen H; Key Laboratory of Translational Tumor Medicine in Fujian Province, Putian University, School of Basic Medicine Science, Putian, 351100, Fujian, China.
  • Pan S; Key Laboratory of Translational Tumor Medicine in Fujian Province, Putian University, School of Basic Medicine Science, Putian, 351100, Fujian, China. plar000@163.com.
Arch Gynecol Obstet ; 310(2): 1255-1264, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38814453
ABSTRACT

BACKGROUND:

Gestational diabetes mellitus (GDM) poses significant health risks for both mothers and children, contributing to long-term complications such as type 2 diabetes and cardiovascular disease. This study explores the potential of microRNAs (miRNAs) as biomarkers for GDM by analyzing peripheral blood samples from GDM patients.

METHOD:

Ten samples, including peripheral blood from 5 GDM patients and 5 controls, were collected to perform the RNA sequencing analysis. Differentially expressed miRNAs were further validated by quantitative real-time polymerase chain reaction.

RESULTS:

A total of 2287 miRNAs were identified, 229 of which showed differential expression. Validation by qRT-PCR confirmed significant up-regulation of miR-5193, miR-5003-3p, miR-3127-5p, novel-miR-96, miR-6734-5p, and miR-122-5p, while miR-10395-3p was down-regulated. Bioinformatics analyses revealed the involvement of these miRNAs in pathways associated with herpes simplex virus 1 infection.

CONCLUSION:

This study provides insights into the differential expression of miRNAs in GDM patients and their potential roles in disease pathogenesis. It suggests that the differentially expressed miRNAs could serve as potential biomarkers for GDM, shedding light on the complex molecular mechanisms involved.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores / Diabetes Gestacional / Análisis de Secuencia de ARN / MicroARNs Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: Arch Gynecol Obstet Asunto de la revista: GINECOLOGIA / OBSTETRICIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores / Diabetes Gestacional / Análisis de Secuencia de ARN / MicroARNs Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: Arch Gynecol Obstet Asunto de la revista: GINECOLOGIA / OBSTETRICIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania