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Analysis of global Aeromonas caviae genomes revealed that strains carrying T6SS are more common in human gastroenteritis than in environmental sources and are often phylogenetically related.
Chong, Sarah K T; Liu, Fang; Yuwono, Christopher; Tay, Alfred Chin Yen; Wehrhahn, Michael C; Riordan, Stephen M; Liu, Lu; Zhang, Li.
Afiliación
  • Chong SKT; School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
  • Liu F; School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
  • Yuwono C; School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
  • Tay ACY; Helicobacter Research Laboratory, School of Pathology and Laboratory Medicine, Marshall Centre for Infectious Diseases Research and Training, University of Western Australia, Perth, Australia.
  • Wehrhahn MC; Douglass Hanly Moir Pathology, Macquarie Park, New South Wales, Australia.
  • Riordan SM; Gastrointestinal and Liver Unit, Prince of Wales Hospital, University of New South Wales, Sydney, Australia.
  • Liu L; School of Biomedical Sciences, University of New South Wales, Sydney, Australia.
  • Zhang L; School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
Microb Genom ; 10(5)2024 May.
Article en En | MEDLINE | ID: mdl-38814176
ABSTRACT
Aeromonas caviae is an emerging human enteric pathogen. However, the genomic features and virulence genes of A. caviae strains from human gastroenteritis and other sources have not been fully elucidated. Here, we conducted a genomic analysis of 565 global A. caviae strains isolated from different sources, including 261 strains isolated from faecal samples of gastroenteritis patients, of which 18 genomes were sequenced in this study. The presence of bacterial virulence genes and secretion systems in A. caviae strains from different sources was compared, and the phylogenetic relationship of A. caviae strains was assessed based on the core genome. The complete genome of A. caviae strain A20-9 isolated from a gastroenteritis patient was obtained in this study, from which 300 putative virulence factors and a T4SS-encoding plasmid, pAC, were identified. Genes encoding T4SS were also identified in a novel genomic island, ACI-1, from other T4SS-positive strains. The prevalence of T4SS was significantly lower in A. caviae strains from gastroenteritis patients than in environmental strains (3 %, P<0.0001 vs 14 %, P<0.01). Conversely, the prevalence of T6SS was significantly higher in A. caviae strains isolated from gastroenteritis patients than in environmental strains (25 %, P<0.05 vs 13  %, P<0.01). Four phylogenetic clusters were formed based on the core genome of 565 A. caviae strains, and strains carrying T6SS often showed close phylogenetic relationships. T3SS, aerolysin and thermostable cytotonic enterotoxin were absent in all 565 A. caviae strains. Our findings provide novel information on the genomic features of A. caviae and suggest that T6SS may play a role in A. caviae-induced human gastroenteritis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Genoma Bacteriano / Factores de Virulencia / Aeromonas caviae / Gastroenteritis Límite: Humans Idioma: En Revista: Microb Genom Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Genoma Bacteriano / Factores de Virulencia / Aeromonas caviae / Gastroenteritis Límite: Humans Idioma: En Revista: Microb Genom Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido