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Partial Acquisition of Spectral Projections Accelerates Four-dimensional Spectral-spatial EPR Imaging for Mouse Tumor Models: A Feasibility Study.
Oba, Misa; Taguchi, Mai; Kudo, Yohei; Yamashita, Koya; Yasui, Hironobu; Matsumoto, Shingo; Kirilyuk, Igor A; Inanami, Osamu; Hirata, Hiroshi.
Afiliación
  • Oba M; Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology, Hokkaido University, North 14, West 9, Kita-ku, Sapporo, 060-0814, Japan.
  • Taguchi M; Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology, Hokkaido University, North 14, West 9, Kita-ku, Sapporo, 060-0814, Japan.
  • Kudo Y; Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology, Hokkaido University, North 14, West 9, Kita-ku, Sapporo, 060-0814, Japan.
  • Yamashita K; Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, North 18, West 9, Kita-ku, Sapporo, 060-0818, Japan.
  • Yasui H; Laboratory of Radiation Biology, Faculty of Veterinary Medicine, Hokkaido University, North 18, West 9, Kita-ku, Sapporo, 060-0818, Japan.
  • Matsumoto S; Division of Bioengineering and Bioinformatics, Faculty of Information Science and Technology, Hokkaido University, North 14, West 9, Kita-ku, Sapporo, 060-0814, Japan.
  • Kirilyuk IA; N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, 9, Ac. Lavrentieva Ave, Novosibirsk, 630090, Russia.
  • Inanami O; Laboratory of Radiation Biology, Faculty of Veterinary Medicine, Hokkaido University, North 18, West 9, Kita-ku, Sapporo, 060-0818, Japan.
  • Hirata H; Division of Bioengineering and Bioinformatics, Faculty of Information Science and Technology, Hokkaido University, North 14, West 9, Kita-ku, Sapporo, 060-0814, Japan. hhirata@ist.hokudai.ac.jp.
Mol Imaging Biol ; 26(3): 459-472, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38811467
ABSTRACT

PURPOSE:

Our study aimed to accelerate the acquisition of four-dimensional (4D) spectral-spatial electron paramagnetic resonance (EPR) imaging for mouse tumor models. This advancement in EPR imaging should reduce the acquisition time of spectroscopic mapping while reducing quality degradation for mouse tumor models. PROCEDURES EPR spectra under magnetic field gradients, called spectral projections, were partially measured. Additional spectral projections were later computationally synthesized from the measured spectral projections. Four-dimensional spectral-spatial images were reconstructed from the post-processed spectral projections using the algebraic reconstruction technique (ART) and assessed in terms of their image qualities. We applied this approach to a sample solution and a mouse Hs766T xenograft model of human-derived pancreatic ductal adenocarcinoma cells to demonstrate the feasibility of our concept. The nitroxyl radical imaging agent 2H,15N-DCP was exogenously infused into the mouse xenograft model.

RESULTS:

The computation code of 4D spectral-spatial imaging was tested with numerically generated spectral projections. In the linewidth mapping of the sample solution, we achieved a relative standard uncertainty (standard deviation/| mean |) of 0.76 µT/45.38 µT = 0.017 on the peak-to-peak first-derivative EPR linewidth. The qualities of the linewidth maps and the effect of computational synthesis of spectral projections were examined. Finally, we obtained the three-dimensional linewidth map of 2H,15N-DCP in a Hs766T tumor-bearing leg in vivo.

CONCLUSION:

We achieved a 46.7% reduction in the acquisition time of 4D spectral-spatial EPR imaging without significantly degrading the image quality. A combination of ART and partial acquisition in three-dimensional raster magnetic field gradient settings in orthogonal coordinates is a novel approach. Our approach to 4D spectral-spatial EPR imaging can be applied to any subject, especially for samples with less variation in one direction.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estudios de Factibilidad Límite: Animals / Humans Idioma: En Revista: Mol Imaging Biol Asunto de la revista: BIOLOGIA MOLECULAR / DIAGNOSTICO POR IMAGEM Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estudios de Factibilidad Límite: Animals / Humans Idioma: En Revista: Mol Imaging Biol Asunto de la revista: BIOLOGIA MOLECULAR / DIAGNOSTICO POR IMAGEM Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos