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A Thermoresponsive injectable drug delivery system of chitosan/ß-glycerophosphate with gellan gum/alginate microparticles.
Carrêlo, H; Jiménez-Rosado, M; Vieira, Tânia; Da Rosa, Rafaela R; Perez-Puyana, Victor M; Silva, Jorge Carvalho; Romero, A; Borges, J P; Soares, Paula I P.
Afiliación
  • Carrêlo H; CENIMAT/i3N, Department of Materials Science, NOVA School of Science and Technology (NOVA FCT), Campus de Caparica, 2829-516 Caparica, Portugal. Electronic address: hnccarrelo@gmail.com.
  • Jiménez-Rosado M; Department of Applied Chemistry and Physics, Universidad de León, 24007 León, Spain.
  • Vieira T; CENIMAT/i3N, Department of Physics, NOVA School of Science and Technology (NOVA FCT), Campus de Caparica, 2829-516 Caparica, Portugal.
  • Da Rosa RR; CENIMAT/i3N, Department of Materials Science, NOVA School of Science and Technology (NOVA FCT), Campus de Caparica, 2829-516 Caparica, Portugal.
  • Perez-Puyana VM; Department of Chemical Engineering, Universidad de Sevilla, 41012 Sevilla, Spain.
  • Silva JC; CENIMAT/i3N, Department of Physics, NOVA School of Science and Technology (NOVA FCT), Campus de Caparica, 2829-516 Caparica, Portugal. Electronic address: jcs@fct.unl.pt.
  • Romero A; Department of Chemical Engineering, Universidad de Sevilla, 41012 Sevilla, Spain. Electronic address: alromero@us.es.
  • Borges JP; CENIMAT/i3N, Department of Materials Science, NOVA School of Science and Technology (NOVA FCT), Campus de Caparica, 2829-516 Caparica, Portugal. Electronic address: jpb@fct.unl.pt.
  • Soares PIP; CENIMAT/i3N, Department of Materials Science, NOVA School of Science and Technology (NOVA FCT), Campus de Caparica, 2829-516 Caparica, Portugal. Electronic address: pi.soares@fct.unl.pt.
Int J Biol Macromol ; 271(Pt 1): 131981, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38811317
ABSTRACT
The development of new Drug Delivery Systems (DDS) by incorporating microparticles within hydrogels can prolong the release rate of drugs and/or other bioactive agents. In this study, we combined gellan gum/alginate microparticles within a thermoresponsive chitosan (Ch) hydrogel with ß-Glycerophosphate (ß-GP), designing the system to be in the sol state at 21 °C and in the gel state at 37 °C to enable the injectability of the system. The system was in the sol state between 10 °C and 21 °C. Higher concentrations of ß-GP (0, 2, 3, 4, 5 w/v%) and microparticles (0, 2 and 5 w/v%) allowed a faster sol-gel transition with higher mechanical strength at 37 °C. However, the sol-gel transition was not instantaneous. The release profile of methylene blue (MB) from the microparticles was significantly affected by their incorporation in Ch/ß-GP hydrogels, only allowing the release of 60-70 % of MB for 6 days, while the microparticles alone released all the MB in 48 h. The proposed system did not present cytotoxicity to VERO cell lines as a preliminary assay, with the Ch/ß-GP/GGAlg having >90 % of cellular viability. The proposed Ch/ß-GP system proved to have a delaying effect on drug release and biocompatible properties, being a promising future DDS.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos Bacterianos / Quitosano / Alginatos / Glicerofosfatos Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos Bacterianos / Quitosano / Alginatos / Glicerofosfatos Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos