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Effect of a novel siRNA delivery system, siRNA ternary complex, on melanoma lung metastasis.
Kurosaki, Tomoaki; Ueda, Yuki; Kato, Yuka; Nakashima, Mikiro; Kitahara, Takashi; Sasaki, Hitoshi; Kodama, Yukinobu.
Afiliación
  • Kurosaki T; Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
  • Ueda Y; Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
  • Kato Y; Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
  • Nakashima M; Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
  • Kitahara T; Pharmacy Department, Yamaguchi University Hospital, Yamaguchi, Japan.
  • Sasaki H; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
  • Kodama Y; Department of Hospital Pharmacy, Nagasaki University Hospital, Nagasaki, Japan.
J Drug Target ; 32(7): 848-854, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38809595
ABSTRACT
In this study, we determined effects of an anionic siRNA delivery vector, siRNA ternary complex, which is constructed with biodegradable dendrigraft poly-L-lysine (DGL) and γ-polyglutamic acid (γ-PGA) on the melanoma cells and melanoma lung metastasis. The siRNA ternary complex showed high cellular uptake and silencing effect in melanoma cell line B16-F10/Luc cells. After intravenous administration of the siRNA ternary complex, high silencing effect was also observed in the lung of B16-F10/Luc melanoma metastasis model mice. Therefore, we applied vascular endothelial growth factor (VEGF)-siRNA on the siRNA ternary complex and determined the effect on the melanoma lung metastasis. The siRNA ternary complex containing VEGF-siRNA reduced VEGF protein levels significantly in in vitro and in vivo, and the complex successfully inhibited melanoma lung metastasis. This biodegradable and effective siRNA delivery vector, siRNA ternary complex, could be available for clinical trials.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Poliglutámico / Polilisina / Melanoma Experimental / ARN Interferente Pequeño / Factor A de Crecimiento Endotelial Vascular / Neoplasias Pulmonares Límite: Animals Idioma: En Revista: J Drug Target Asunto de la revista: FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Poliglutámico / Polilisina / Melanoma Experimental / ARN Interferente Pequeño / Factor A de Crecimiento Endotelial Vascular / Neoplasias Pulmonares Límite: Animals Idioma: En Revista: J Drug Target Asunto de la revista: FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido