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Plasmodium falciparum AMA1 and CSP antigen diversity in parasite isolates from southern Ghana.
Kusi, Kwadwo A; Amoah, Linda E; Acquah, Festus Kojo; Ennuson, Nana Aba; Frempong, Abena F; Ofori, Ebenezer A; Akyea-Mensah, Kwadwo; Kyei-Baafour, Eric; Osei, Frank; Frimpong, Augustina; Singh, Susheel K; Theisen, Michael; Remarque, Edmond J; Faber, Bart W; Belmonte, Maria; Ganeshan, Harini; Huang, Jun; Villasante, Eileen; Sedegah, Martha.
Afiliación
  • Kusi KA; Department of Immunology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana.
  • Amoah LE; Department of Immunology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana.
  • Acquah FK; Department of Immunology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana.
  • Ennuson NA; Department of Immunology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana.
  • Frempong AF; Department of Immunology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana.
  • Ofori EA; Department of Immunology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana.
  • Akyea-Mensah K; Department of Immunology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana.
  • Kyei-Baafour E; Department of Immunology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana.
  • Osei F; Department of Immunology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana.
  • Frimpong A; Department of Immunology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana.
  • Singh SK; Center for Medical Parasitology at the Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
  • Theisen M; Department of Congenital Diseases, Statens Serum Institut, Copenhagen, Denmark.
  • Remarque EJ; Center for Medical Parasitology at the Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.
  • Faber BW; Department of Congenital Diseases, Statens Serum Institut, Copenhagen, Denmark.
  • Belmonte M; Department of Parasitology, Biomedical Primate Research Center, Rijswijk, Netherlands.
  • Ganeshan H; Department of Parasitology, Biomedical Primate Research Center, Rijswijk, Netherlands.
  • Huang J; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, United States.
  • Villasante E; Malaria Department, Naval Medical Research Command, Silver Spring, MD, United States.
  • Sedegah M; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, United States.
Front Cell Infect Microbiol ; 14: 1375249, 2024.
Article en En | MEDLINE | ID: mdl-38808064
ABSTRACT

Introduction:

Diversity in malarial antigens is an immune evasion mechanism that gives malaria parasites an edge over the host. Immune responses against one variant of a polymorphic antigen are usually not fully effective against other variants due to altered epitopes. This study aimed to evaluate diversity in the Plasmodium falciparum antigens apical membrane antigen 1 (PfAMA1) and circumsporozoite protein (PfCSP) from circulating parasites in a malaria-endemic community in southern Ghana and to determine the effects of polymorphisms on antibody response specificity.

Methods:

The study involved 300 subjects, whose P. falciparum infection status was determined by microscopy and PCR. Diversity within the two antigens was evaluated by msp2 gene typing and molecular gene sequencing, while the host plasma levels of antibodies against PfAMA1, PfCSP, and two synthetic 24mer peptides from the conserved central repeat region of PfCSP, were measured by ELISA.

Results:

Of the 300 subjects, 171 (57%) had P. falciparum infection, with 165 of the 171 (96.5%) being positive for either or both of the msp2 allelic families. Gene sequencing of DNA from 55 clonally infected samples identified a total of 56 non-synonymous single nucleotide polymorphisms (SNPs) for the Pfama1 gene and these resulted in 44 polymorphic positions, including two novel positions (363 and 365). Sequencing of the Pfcsp gene from 69 clonal DNA samples identified 50 non-synonymous SNPs that resulted in 42 polymorphic positions, with half (21) of these polymorphic positions being novel. Of the measured antibodies, only anti-PfCSP antibodies varied considerably between PCR parasite-positive and parasite-negative persons.

Discussion:

These data confirm the presence of a considerable amount of unique, previously unreported amino acid changes, especially within PfCSP. Drivers for this diversity in the Pfcsp gene do not immediately seem apparent, as immune pressure will be expected to drive a similar level of diversity in the Pfama1 gene.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Anticuerpos Antiprotozoarios / Proteínas Protozoarias / Malaria Falciparum / Proteínas de la Membrana / Antígenos de Protozoos Límite: Adolescent / Adult / Child / Child, preschool / Female / Humans / Male / Middle aged País/Región como asunto: Africa Idioma: En Revista: Front Cell Infect Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Ghana Pais de publicación: Suiza
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Anticuerpos Antiprotozoarios / Proteínas Protozoarias / Malaria Falciparum / Proteínas de la Membrana / Antígenos de Protozoos Límite: Adolescent / Adult / Child / Child, preschool / Female / Humans / Male / Middle aged País/Región como asunto: Africa Idioma: En Revista: Front Cell Infect Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Ghana Pais de publicación: Suiza