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CRISPR/Cas-based colorimetric biosensors: a promising tool for the diagnosis of bacterial foodborne pathogens in food products.
Saleh, Ebraheem Abdu Musad; Ali, Eyhab; Muxamadovna, Giyazova Malika; Kassem, Asmaa F; Kaur, Irwanjot; Kumar, Abhinav; Jabbar, Hijran Sanaan; Alwaily, Enas R; Elawady, Ahmed; Omran, Alaa A.
Afiliación
  • Saleh EAM; Department of Chemistry, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj, 11942, Saudi Arabia. abdu65.musadsaleh@gmail.com.
  • Ali E; Al-Zahraa University for Women, Karbala, Iraq.
  • Muxamadovna GM; Assistant Teacher of the Physiology Department, Bukhara State Medical Institute, Bukhara, Uzbekistan.
  • Kassem AF; Department of Chemistry, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj, 11942, Saudi Arabia.
  • Kaur I; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Cairo 12622, Egypt.
  • Kumar A; Department of Biotechnology and Genetics, Jain (Deemed-to-be) University, Bengaluru, Karnataka-560069, India.
  • Jabbar HS; Department of Allied Healthcare and Sciences, Vivekananda Global University, Jaipur, Rajasthan-303012, India.
  • Alwaily ER; Department of Nuclear and Renewable Energy, Ural Federal University Named After the First President of Russia Boris Yeltsin, Yekaterinburg 620002, Russia.
  • Elawady A; Department of Chemistry, College of Science, Salahaddin University-Erbil, Erbil, Kurdistan Region, Iraq.
  • Omran AA; Microbiology Research Group, College of Pharmacy, Al-Ayen University, Thi-Qar, Iraq.
Anal Methods ; 16(22): 3448-3463, 2024 Jun 06.
Article en En | MEDLINE | ID: mdl-38804827
ABSTRACT
Some physical phenomena and various chemical substances newly introduced in nanotechnology have allowed scientists to develop valuable devices in the field of food sciences. Regarding such progress, the identification of foodborne pathogenic microorganisms is an imperative subject nowadays. These bacterial species have been found to cause severe health impacts after food ingestion and can result in high mortality in acute cases. The rapid detection of foodborne bacterial species at low concentrations is in high demand in recent diagnostics. CRISPR/Cas-mediated biosensors possess the potential to overcome several challenges in classical assays such as complex pretreatments, long turnaround time, and insensitivity. Among them, colorimetric nanoprobes based on the CRISPR strategy afford promising devices for POCT (point-of-care testing) since they can be visualized with the naked eye and do not require diagnostic apparatus. In this study, we briefly classify and discuss the working principles of the different CRISPR/Cas protein agents that have been employed in biosensors so far. We assess the current status of the CRISPR system, specifically focusing on colorimetric biosensing platforms. We discuss the utilization of each Cas effector in the detection of foodborne pathogens and examine the restrictions of the existing technology. The challenges and future opportunities are also indicated and addressed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Colorimetría / Sistemas CRISPR-Cas / Microbiología de Alimentos / Enfermedades Transmitidas por los Alimentos Límite: Humans Idioma: En Revista: Anal Methods Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Colorimetría / Sistemas CRISPR-Cas / Microbiología de Alimentos / Enfermedades Transmitidas por los Alimentos Límite: Humans Idioma: En Revista: Anal Methods Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Reino Unido