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Two-photon imaging of excitatory and inhibitory neural response to infrared neural stimulation.
Fu, Peng; Liu, Yin; Zhu, Liang; Wang, Mengqi; Yu, Yuan; Yang, Fen; Zhang, Weijie; Zhang, Hequn; Shoham, Shy; Roe, Anna Wang; Xi, Wang.
Afiliación
  • Fu P; Second Affiliated Hospital, Zhejiang University, Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Hangzhou, China.
  • Liu Y; Second Affiliated Hospital, Zhejiang University, Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Hangzhou, China.
  • Zhu L; KU Leuven Medical School, Laboratory for Neuro- and Psychophysiology, Department of Neurosciences, Leuven, Belgium.
  • Wang M; Second Affiliated Hospital, Zhejiang University, Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Hangzhou, China.
  • Yu Y; Zhejiang University, College of Biomedical Engineering and Instrument Science, Key Laboratory of Biomedical Engineering of Ministry of Education, Hangzhou, China.
  • Yang F; Second Affiliated Hospital, Zhejiang University, Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Hangzhou, China.
  • Zhang W; Second Affiliated Hospital, Zhejiang University, Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Hangzhou, China.
  • Zhang H; Second Affiliated Hospital, Zhejiang University, Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Hangzhou, China.
  • Shoham S; Zhejiang University, College of Biomedical Engineering and Instrument Science, Key Laboratory of Biomedical Engineering of Ministry of Education, Hangzhou, China.
  • Roe AW; Second Affiliated Hospital, Zhejiang University, Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Hangzhou, China.
  • Xi W; NYU Langone Health, Department of Ophthalmology and Tech4Health and Neuroscience Institutes, New York, New York, United States.
Neurophotonics ; 11(2): 025003, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38800606
ABSTRACT

Significance:

Pulsed infrared neural stimulation (INS, 1875 nm) is an emerging neurostimulation technology that delivers focal pulsed heat to activate functionally specific mesoscale networks and holds promise for clinical application. However, little is known about its effect on excitatory and inhibitory cell types in cerebral cortex.

Aim:

Estimates of summed population neuronal response time courses provide a potential basis for neural and hemodynamic signals described in other studies.

Approach:

Using two-photon calcium imaging in mouse somatosensory cortex, we have examined the effect of INS pulse train application on hSyn neurons and mDlx neurons tagged with GCaMP6s.

Results:

We find that, in anesthetized mice, each INS pulse train reliably induces robust response in hSyn neurons exhibiting positive going responses. Surprisingly, mDlx neurons exhibit negative going responses. Quantification using the index of correlation illustrates responses are reproducible, intensity-dependent, and focal. Also, a contralateral activation is observed when INS applied.

Conclusions:

In sum, the population of neurons stimulated by INS includes both hSyn and mDlx neurons; within a range of stimulation intensities, this leads to overall excitation in the stimulated population, leading to the previously observed activations at distant post-synaptic sites.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Neurophotonics Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Neurophotonics Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos