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Anti-metastatic Effect of Heliotropium bacciferum on MCF-7 Human Breast Cancer Cell Line.
R, Gauranga; Suresh, Vasugi; Selvamani, Muthamizh; Jayaraman, Selvaraj; Hussein, Mohammed Asif.
Afiliación
  • R G; Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Science (SIMATS) Saveetha University, Chennai, IND.
  • Suresh V; Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Science (SIMATS) Saveetha University, Chennai, IND.
  • Selvamani M; Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Science (SIMATS) Saveetha University, Chennai, IND.
  • Jayaraman S; Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Science (SIMATS) Saveetha University, Chennai, IND.
  • Hussein MA; Department of Physiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Science (SIMATS) Saveetha University, Chennai, IND.
Cureus ; 16(4): e58939, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38800305
ABSTRACT
Background Heliotropium bacciferum, often known as wild heliotrope or wild quailplant, is a flowering plant from the borage family. This study examines the anti-metastatic impact of H. bacciferum on Michigan Cancer Foundation-7 (MCF-7) breast cancer cells and its ability to disrupt signaling pathways. Aim To explore the anti-metastatic effect of H. bacciferum on the MCF-7 breast cancer cell line. Materials and methods For this research, MCF-7 breast cancer cells were used. Cells were cultured and subjected to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, as well as gene expression analysis for glycogen synthase kinase 3 beta (GSK3ß), wingless-related integration site 2 (Wnt2), and ß-catenin. The plant extract was tested to determine if it successfully blocked the signalling pathway or not.  Results The MTT test was performed to study the cytotoxic impact of H. bacciferum. At an increasing concentration of 100 µg/mL, the extract inhibited growth by 55%, whereas at 150 µg/mL, it inhibited growth by 52.5%. Maximum inhibition was seen at 150 µg/mL. H. bacciferum suppressed the GSK3ß and Wnt2 signaling pathways in MCF-7 breast cancer cell lines, acting as an anti-metastatic and anticancer agent. The heliotrine compound in H. bacciferum showed high binding energy to metastatic targets such as GSK3ß, Wnt2, and ß-catenin. Moreover, chemical absorption, distribution, metabolism, excretion, and toxicity (ADMET) properties also support the study. Conclusion In this study, we can infer that H. bacciferum has a favourable anticancer impact on MCF-7 breast cancer cell lines and may be utilised as an anticancer drug against breast cancer cells. It can also be further evaluated for different breast cancers and cell lines.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Cureus Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Cureus Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos