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Comparison of lipopolysaccharide-mediated peripheral blood mononuclear cell activation between Brahman and Brahman × Thai native crossbreed cattle.
Srinontong, Piyarat; Aengwanich, Worapol; Somphon, Sattabongkod; Khonwai, Siriyakorn; Nitsinsakul, Thanasorn; Wu, Zhiliang; Chalalai, Thanyakorn; Saraphol, Bhuripit; Srisanyong, Wilasinee.
Afiliación
  • Srinontong P; Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
  • Aengwanich W; Bioveterinary Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
  • Somphon S; Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
  • Khonwai S; Stress and Oxidative Stress in Animal Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
  • Nitsinsakul T; Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
  • Wu Z; Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
  • Chalalai T; Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
  • Saraphol B; Department of Parasitology and Infectious Diseases, Gifu University Graduate School of Medicine, Gifu 5011194, Japan.
  • Srisanyong W; Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
Vet World ; 17(4): 804-810, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38798282
ABSTRACT
Background and

Aims:

Lipopolysaccharide (LPS) is a robust endotoxin known to activate the immune system in cattle. The objective of this study was to investigate the effect of LPS on the morphology, cell viability, malondialdehyde (MDA), nitric oxide (NO), and total antioxidant capacity (TAC) of peripheral blood mononuclear cells (PBMCs) in Brahman and Brahman × Thai native crossbreed cattle. Materials and

Methods:

PBMCs were isolated from Brahman and Brahman × Thai native crossbreed cattle and treated with 0, 0.1, 1, and 10 µg/mL Escherichia coli LPS, respectively. Morphological changes in PBMCs were assessed at 24 and 48 h. In addition, we measured PBMC cell viability, MDA, NO, and TAC.

Results:

LPS stimulation caused cell deformation and partial PBMC area enlargement, but there were no differences between Brahman and Brahman × Thai native crossbreed cattle. Stimulation at all levels did not affect the viability of PBMCs (p > 0.05). MDA and NO levels were significantly higher in Brahman cattle than in Brahman Thai native crossbred cattle (p < 0.05). TAC was significantly higher in Brahman × Thai native crossbred cattle than in Brahman cattle (p < 0.05).

Conclusion:

Immune cells of crossbreed cattle have a higher activation response to LPS than those of purebred cattle, and native crossbreed beef cattle have a higher antioxidant capacity than purebred beef cattle. This result may explain why hybrid cattle of indigenous breeds are more resistant to disease than purebred cattle.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet World Año: 2024 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: India
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet World Año: 2024 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: India