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Platelet microparticles influence gene expression and modulate biological activities of chronic myeloid leukemia cells (K562).
Nikravesh, Fariba; Mirzaee Khalilabadi, Roohollah; Farsinejad, Alireza; Mardani Valandani, Hajar.
Afiliación
  • Nikravesh F; Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Medical University Campus, Haft-Bagh Highway, Kerman, Iran.
  • Mirzaee Khalilabadi R; Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Medical University Campus, Haft-Bagh Highway, Kerman, Iran.
  • Farsinejad A; Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Medical University Campus, Haft-Bagh Highway, Kerman, Iran.
  • Mardani Valandani H; Cell Therapy and Regenerative Medicine Comprehensive Center, Kerman University of Medical Sciences, Medical University Campus, Haft-Bagh Highway, Kerman, Iran.
Mol Biol Rep ; 51(1): 676, 2024 May 25.
Article en En | MEDLINE | ID: mdl-38796661
ABSTRACT

BACKGROUND:

The current understanding emphasizes the intricate interplay between the Leukemic cell and its environment. Platelet-derived microparticles play a crucial role in facilitating intercellular communication and contribute to the complex landscape of cancer pathology. This study aimed to investigate the influence of platelet-derived microparticles on cell proliferation, apoptosis, and the expression of key genes, including P53, P21, Cyclin D1, Bax, and Bcl-2, within the context of a chronic myeloid leukemia cell line (K562). METHODS AND

RESULTS:

Platelet-derived microparticles were obtained through centrifugation at various speeds, and their concentration was quantified using the BCA assay. To determine the size and immunophenotypic characteristics of the PMPs, both the DLS technique and flow cytometry were employed. Cell proliferation was assessed using the MTT assay and hemocytometer, and cell cycle analysis was conducted through DNA content evaluation. Real-time PCR was utilized for gene expression analysis of Bax, Bcl-2, Cyclin D1, P53, and P21. Flow cytometry was employed to examine cell apoptosis. The findings revealed that platelet-derived microparticles have the ability to decrease proliferation of the K562 cell line, while not exerting an impact on apoptosis and cell cycle progression. Analysis through real-time PCR indicated an upregulation in the gene expression of P53, P21, and Bcl-2, accompanied by a downregulation in Bax and Cyclin D1.

CONCLUSION:

This investigation sheds light on the intricate relationship between chronic myeloid leukemia and its microenvironment, particularly the involvement of platelet-derived microparticles. The study underscores the potential of platelet-derived microparticles to influence cell behavior and gene expression, providing a deeper understanding of their role in CML and its therapeutic implications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaquetas / Leucemia Mielógena Crónica BCR-ABL Positiva / Apoptosis / Proliferación Celular / Micropartículas Derivadas de Células Límite: Humans Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaquetas / Leucemia Mielógena Crónica BCR-ABL Positiva / Apoptosis / Proliferación Celular / Micropartículas Derivadas de Células Límite: Humans Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos