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Impact of processing on the sensitising capacity and cross-reactivity of cow's and camel milk proteins in a Brown Norway rat study.
Maryniak, Natalia Zofia; Mancino, Matteo; Sztuk, Tiffany Kirkaldy Spaanager; Gao, Yumei; Sancho, Ana Isabel; Hansen, Egon Bech; Bøgh, Katrine Lindholm.
Afiliación
  • Maryniak NZ; National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.
  • Mancino M; National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.
  • Sztuk TKS; National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.
  • Gao Y; Ausnutria Dairy (China) Co., Ltd., Changsha, China.
  • Sancho AI; National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.
  • Hansen EB; National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.
  • Bøgh KL; National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark. Electronic address: kalb@food.dtu.dk.
Food Chem Toxicol ; 189: 114761, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38796088
ABSTRACT
Infant formulas based on hydrolysed cow's milk proteins are used when breastfeeding is not feasible in cow's milk allergic infants. Camel milk has been shown to be well-tolerated by the majority of children with cow's milk allergy (CMA) and may be a substitute in management of CMA. Here we aimed to evaluate the impact of processing on immunogenicity, sensitising, antibody-binding and cross-reactive capacity of cow's and camel milk. Cow's and camel milk were processed by means of enzyme hydrolysis or heat treatment. Brown Norway rats were immunised with PBS, non-processed, enzyme hydrolysed or heat-treated cow's or camel milk. In vivo tests were performed for evaluation of clinical signs. Blood and faecal samples were analysed for levels and specificity of antibody responses. Cow's and camel milk showed similar sensitising capacity. Processing decreased the sensitising capacity of cow's milk, yet only enzyme hydrolysis but not heat treatment decreased the sensitising capacity of camel milk. Processing affected the specificity of antibodies raised in the rats, though the effect differed between cow's and camel milk. The study showed a low cross-reactivity between cow's and camel milk, which was decreased with processing, suggesting that processing of camel milk may improve its usefulness in CMA management.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Camelus / Hipersensibilidad a la Leche / Reacciones Cruzadas / Leche / Proteínas de la Leche Límite: Animals Idioma: En Revista: Food Chem Toxicol Año: 2024 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Camelus / Hipersensibilidad a la Leche / Reacciones Cruzadas / Leche / Proteínas de la Leche Límite: Animals Idioma: En Revista: Food Chem Toxicol Año: 2024 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido