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Fabrication and characterization of physically crosslinked alginate/chitosan-based hydrogel loaded with neomycin for the treatment of skin infections caused by Staphylococcus aureus.
Silva, Lucas Dos Santos; Vila Nova, Beatriz Gomes; Sousa, Carlos Eduardo Morais de; Silva, Raphael Guedes; Carvalho, Layse Ribeiro de Sousa; Silva, Izadora Souza Soeiro; Moreira, Pedro Henrique de Aguiar; Cardenas, Andres Felipe Millan; Monteiro, Cristina de Andrade; Tofanello, Aryane; Garcia, Wanius; Teixeira, Claudener Souza; Nascimento da Silva, Luís Cláudio.
Afiliación
  • Silva LDS; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil.
  • Vila Nova BG; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil.
  • Sousa CEM; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil.
  • Silva RG; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil.
  • Carvalho LRS; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil.
  • Silva ISS; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil.
  • Moreira PHA; Laboratório de Odontologia, Universidade CEUMA, São Luís 65075-120, MA, Brazil.
  • Cardenas AFM; Laboratório de Odontologia, Universidade CEUMA, São Luís 65075-120, MA, Brazil.
  • Monteiro CA; Laboratório de Pesquisa e Estudo em Microbiologia, Instituto Federal de Educação, Ciência e Tecnologia do Maranhão (IFMA), São Luís 65030-005, Brazil.
  • Tofanello A; Center for Advanced Graphene, Nanomaterials and Nanotechnology Research (MackGraphe), Universidade Presbiteriana Mackenzie, SP, Brazil; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, SP, Brazil.
  • Garcia W; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC, SP, Brazil.
  • Teixeira CS; Centro de Ciências Agrárias e da Biodiversidade, Universidade Federal do Cariri, CE, Brazil.
  • Nascimento da Silva LC; Laboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís 65075-120, MA, Brazil. Electronic address: luiscn.silva@ceuma.com.
Int J Biol Macromol ; 271(Pt 1): 132577, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38795887
ABSTRACT
Staphylococcus aureus is a pathogen widely involved in wound infection due to its ability to release several virulence factors that impair the skin healing process, as well as its mechanism of drug resistance. Herein, sodium alginate and chitosan were combined to produce a hydrogel for topical delivery of neomycin to combat S. aureus associated with skin complications. The hydrogel was formulated by combining sodium alginate (50 mg/mL) and chitosan (50 mg/mL) solutions in a ratio of 91 (HBase). Neomycin was added to HBase to achieve a concentration of 0.4 mg/mL (HNeo). The incorporation of neomycin into the product was confirmed by scanning electron microscopy, FTIR and TGA analysis. The hydrogels produced are homogeneous, have a high swelling capacity, and show biocompatibility using erythrocytes and fibroblasts as models. The formulations showed physicochemical and pharmacological stability for 60 days at 4 ± 2 °C. HNeo totally inhibited the growth of S. aureus after 4 h. The antimicrobial effects were confirmed using ex vivo (porcine skin) and in vivo (murine) wound infection models. Furthermore, the HNeo-treated mice showed lower severity scores than those treated with HBase. Taken together, the obtained results present a new low-cost bioproduct with promising applications in treating infected wounds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus / Neomicina / Hidrogeles / Quitosano / Alginatos / Antibacterianos Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus / Neomicina / Hidrogeles / Quitosano / Alginatos / Antibacterianos Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos