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Comparison of Five Serological Methods for the Detection of West Nile Virus Antibodies.
Girl, Philipp; Euringer, Kathrin; Coroian, Mircea; Mihalca, Andrei Daniel; Borde, Johannes P; Dobler, Gerhard.
Afiliación
  • Girl P; Bundeswehr Institute of Microbiology, 80937 Munich, Germany.
  • Euringer K; Central Institute of the Bundeswehr Medical Service Munich, 85748 Garching, Germany.
  • Coroian M; Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, 80539 Munich, Germany.
  • Mihalca AD; Bundeswehr Institute of Microbiology, 80937 Munich, Germany.
  • Borde JP; Division of Infectious Diseases, Department of Medicine II, University Medical Centre Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg im Breisgau, Germany.
  • Dobler G; Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372 Cluj-Napoca, Romania.
Viruses ; 16(5)2024 05 15.
Article en En | MEDLINE | ID: mdl-38793670
ABSTRACT
The West Nile Virus (WNV), a member of the family Flaviviridae, is an emerging mosquito-borne flavivirus causing potentially severe infections in humans and animals involving the central nervous system (CNS). Due to its emerging tendency, WNV now occurs in many areas where other flaviviruses are co-occurring. Cross-reactive antibodies with flavivirus infections or vaccination (e.g., tick-borne encephalitis virus (TBEV), Usutu virus (USUV), yellow fever virus (YFV), dengue virus (DENV), Japanese encephalitis virus (JEV)) therefore remain a major challenge in diagnosing flavivirus infections. Virus neutralization tests are considered as reference tests for the detection of specific flavivirus antibodies, but are elaborate, time-consuming and need biosafety level 3 facilities. A simple and straightforward assay for the differentiation and detection of specific WNV IgG antibodies for the routine laboratory is urgently needed. In this study, we compared two commercially available enzyme-linked immunosorbent assays (anti-IgG WNV ELISA and anti-NS1-IgG WNV), a commercially available indirect immunofluorescence assay, and a newly developed in-house ELISA for the detection of WNV-NS1-IgG antibodies. All four tests were compared to an in-house NT to determine both the sensitivity and specificity of the four test systems. None of the assays could match the specificity of the NT, although the two NS1-IgG based ELISAs were very close to the specificity of the NT at 97.3% and 94.6%. The in-house WNV-NS1-IgG ELISA had the best performance regarding sensitivity and specificity. The specificities of the ELISA assays and the indirect immunofluorescence assays could not meet the necessary specificity and/or sensitivity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fiebre del Nilo Occidental / Virus del Nilo Occidental / Ensayo de Inmunoadsorción Enzimática / Sensibilidad y Especificidad / Anticuerpos Antivirales Límite: Animals / Humans Idioma: En Revista: Viruses Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fiebre del Nilo Occidental / Virus del Nilo Occidental / Ensayo de Inmunoadsorción Enzimática / Sensibilidad y Especificidad / Anticuerpos Antivirales Límite: Animals / Humans Idioma: En Revista: Viruses Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza